Abstract
Using the human tumor clonogenic assay technique, the effects of Mitomycin C plus either α-interferon or γ-interferon were studied against five human tumor xenografts serially transplanted into nude mice (three gastric and two colon cancers). When the survival fraction found with the drug combination was smaller than the multiplication of survival fractions with either drug alone, the combined effect was defined as synergism. Similarly, antagonistic effect was defined when the survival fraction of drug combination was larger than the larger one observed in either interferon or Mitomycin C alone. Four out of five human tumor xenografts (three gastric and one colon cancers) showed synergistic effects in combination of α-interferon with Mitomycin C. Though two gastric cancer xenografts exhibited synergistic effects in combination of γ-interferon with Mitomycin C, antagonistic effects, which was not found in combination of α-interferon with Mitomycin C, were observed in one gastric cancer and one colon cancer xenografts.
Similar content being viewed by others
References
Taylor-Papadimitriou J, Balkwill FR. Implications for clinical application of new developments in interferon research. Biochemica et Biophysica Acta 1982; 695: 49–67.
Neidhart JA, Gagen, MM, Young D, Tuttle R, Melink TJ, Ziccarrelli A, Kisner, D. Interferon-α therapy of renal cancer. Cancer Res 1984; 44: 4140–4143.
Kimura K. A cooperative phase I–II study of HLBI in patients with malignant tumors. Gann to Kagakuryoho (Jpn J Cancer Chemother) 1984; 11: 1324–1331. (English Abst.)
Hamburger AW, Salmon SE. Primary bioassay of human tumor stem cells. Science 1977; 197: 461–463.
Hamburger AW, Salmon SE, Kim MB, Trent JM, Soehnlen BJ, Alberts DS, Schmidt HJ. Direct cloning of human ovarian carcinoma cells in agar. Cancer Res 1978; 38: 3438–3444.
Valeriote F, Lin S. Synergistic interaction of anticancer agents: A cellular perspective. Cancer Chemotherapy Reports 1975; 59: 895–900.
Momparler RL.In vitro systems for evaluation of combination chemotherapy. Pharmac Ther 1980; 8: 21–35.
Chirigos MA, Pearson JW. Care of murine leukemia with drug and interferon treatment. J Natl Cancer Inst 1973; 51: 1367–1368.
Gresser I, Maury C, Tovey M. Efficacy of combined interferon cyclophosphamide therapy after diagnosis lymphoma in AKR mice. Eur J Cancer 1978; 14: 97–99.
Balkwill FR, Moodie EM. Positive interactions between human interferon and cyclophosphamide or adriamycin in a human tumor model system. Cancer Res 1984; 44: 904–908.
Schabel FM, Laster WR, Trader MW, Mowshowit SL, Smith GD. Anticancer activity of mouse interferon alone and in combination with cis-dichlorodiammineplatinum II or mitomycin-C against leukemia P388 in mice. Proc Am Ass Cancer Res 1982; p 190.
Noshoh Y, Yoshinaka K, Yamaguchi M, Tani T, Toge T, Niimoto M, Hattori T. Increased cytotoxic effect of various anticancer drugs by α-interferon (HLBI) on human xenografts in nude mice. Gann to Kagakuryoho (Jpn J Cancer Chemother) 1984; 11: 1623–1628. (English Abst.)
Brostrom LA. The combined effect of interferon and methotrexate on human osteosarcoma and lymphoma cell lines. Cancer Lett 1980; 10: 83–90.
Aapro MS, Albert DS, Salmon SE. Interactions of human leukocyte interferon with vinca alkaloids and other chemotherapeutic agents against human tumors in clonogenic assay. Cancer Chemother Pharmacol 1983; 10: 161–166.
Oku T, Imanishi J, Kishida T. Assessment of antitumor cell effect of human leukocyte interferon in combination with anticancer agents by a convenient assay system in monolayer cell culture. Gann 1982; 73: 667–674.
Leandersson T, Lundgren E. Cell growth regulation during density inhibition and interferon treatment. Exp Cell Res 1982; 138: 167–174.
Roos G, Leanderson T, Lundgren E. Interferon-induced cell cycle changes in human hematopoietic cell lines and fresh leukemic cells. Cancer Res 1984; 44: 2358–2362.
Tyring S, Klimpel GR, Fleischmann WR, Baron S. Direct cytolysis by partially-purified preparations of immune interferon. Int J Cancer 1982; 30: 59–64.
Blalock JE, Georgiades JA, Langford MP, Johnson HM. Purified human immune interferon has more potent anticellular activity than fibroblast or leukocyte interferon. Cellular Immunol 1980; 49: 390–394.
Von Hoff DD, Casper J, Bradley E, Sandbach J, Jones D, Makuch R. Association between human tumor colony-forming assay results and response of an individual patient's tumor to chemotherapy. Am J Med 1981; 70: 1027–1032.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hirabayashi, N., Yoshinaka, K., Nosoh, Y. et al. In vitro chemosensitivity tests on human tumor xenografts by clonogenic assay: the combined use of mitomycin C with α-interferon or γ-interferon. The Japanese Journal of Surgery 15, 279–284 (1985). https://doi.org/10.1007/BF02469918
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02469918