Abstract
We designed a new process for culturing pancreatic islets and applied this method to cultures of rat pancreatic islets which had degenerated during preservation by the perfusion-method for 6 hours under the condition of hypothermia and oxygenation. The objective was to determine the extent of the original function. Transplantation of these sotreated islets was also attempted. When pancreatic islets isolated from the ancreas after 6-hour-perfusion were cultured, morphological restoration was apparent within the first 3–4 days. Insulin contents of the culture media renewed every 3 days, ranged from 851 to 1,134 μU/ml/two islets during culture period of 21 days. In the glucose-loading test, insulin secretion of the islets was the same as that of islets in the control experiments. Transplantation of these islets into the portal vein of streptozotocin-induced diabetic rats resulted in a good recovery from the diabetic state.
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Kojima, Y., Nakagawara, G., Takeyama, S. et al. Perfusion preservation of cadaver rat pancreas: II. Culture after perfusion and successful transplantation. The Japanese Journal of Surgery 14, 47–51 (1984). https://doi.org/10.1007/BF02469603
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DOI: https://doi.org/10.1007/BF02469603