Advertisement

The Japanese journal of surgery

, Volume 14, Issue 5, pp 420–431 | Cite as

Effects of trypsin inhibitor on the remant pancreas after 85 percent pancreatectomy in rats

  • Nobuo Baba
  • Takashi Suzuki
  • Takayoshi Tobe
Original Articles

Abstract

Major resection of the pancreas leads to disorders of the endocrine and exocrine pancreas. The effect of a trypsin inhibitor on the remnant pancreas was studied in rats after 85 percent pancreatectomy. Impairments of the glucose elimination rate and the integrated insulin response after 85 percent pancreatectomy were improved by means of oral administration of a synthetic trypsin inhibitor for 4 and 12 weeks. The pancreatic insulin content in the animals treated with trypsin inhibitor for 13 weeks increased to about 1.3 times than that obtained in control animals. The exocrine pancreatic function in 85 percent pancreatectomized rats treated with trypsin inhibitor for 4 weeks and 12 weeks showed substantial improvement as shown by the test with N-benzoyl-L-tyrosil-p-aminobenzoic acid (BT-PABA). The pancreatic amylase, lipase, and protein contents in the animals treated with trypsin inhibitor were increased to 1.9, 1.7 and 2.1-fold, respectively, as compared to control animals for 13 weeks. Histologic examination showed a decrease in abnormal islets of Langerhans, and a tendency toward hypertrophy of the acinar cells. These results suggest that oral administration of a trypsin inhibitor to rats is effective in improving pancreatic endocrine and exocrine functions after 85 percent pancreatectomy.

Key words

trypsin inhibitor pancreatectomy iv GTT PFD-test 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Warren WD, Leite CA, Baumeister F, Pancher RL, Kalser MH. Clinical and metabolic response to radical distal pancreatectomy for chronic pancreatitis. Am J Surg 1967; 113: 77–84.PubMedCrossRefGoogle Scholar
  2. 2.
    Fry WJ, Child CG III. Ninety-five per cent distal pancreatectomy for chronic pancreatitis. Ann Surg 1965; 162: 543–549.PubMedGoogle Scholar
  3. 3.
    Child CG III, Frey CF, Fry WJ. A reappraisal of removal and ninety-five percent of the distal portion of the pancreas. Surg Gynecol Obstet 1969; 129: 49–56.PubMedGoogle Scholar
  4. 4.
    Frey CF, Child CG III, Fry WJ. Pancreatectomy for chronic pancreatitis. Ann Surg 1976; 184: 403–414.PubMedGoogle Scholar
  5. 5.
    Norton L, Eiseman B. Near total pancreatectomy for hemorrhagic pancreatitis. Am J Surg 1974; 127: 191–195.PubMedCrossRefGoogle Scholar
  6. 6.
    Geratz JD. Growth retardation and pancreatic enlargement in rats due to p-aminobenzamidine. Am J Physiol 1968; 214: 594.Google Scholar
  7. 7.
    Rothman SS, Wells H. Selective effects of dietary egg white trypsin inhibitor on pancreatic enzyme secretion. Am J Physiol 1969; 216: 504–509.PubMedGoogle Scholar
  8. 8.
    Melmed RN, Bouchier IAD. A further physiological role for naturally occurring trypsin inhibitors; the evidence for a trophic stimulant of the pancreatic acinar cell. Gut 1969; 10: 973–979.PubMedGoogle Scholar
  9. 9.
    Geratz JD, Hurt JP. Regulation of pancreatic enzyme levels by trypsin inhibitors. Am J Physiol 1970; 219: 705–711.PubMedGoogle Scholar
  10. 10.
    Ihse I, Arnesjo B, Lundquist I. Glucose-induced insulin secretion and pattern of exocrine pancreatic enzymes in the rat following oral and parenteral trypsin inhibitor administration. Scand J Gastroenterol 1974; 9: 719.PubMedGoogle Scholar
  11. 11.
    Ihse I, Arnesjo B, Lundquist, I. Studies on the reversibility of oral trypsin inhibitor induced changes of rat, pancreatic exocrine enzyme activity and insulin secretory capacity. Scand J Gastroenterol 1975; 10: 321–326.PubMedGoogle Scholar
  12. 12.
    Ihse I. Abolishment of oral trypsin inhibitor stimulation of the rat exocrine pancreas after duodenojejunal resection. Scand J Gastroenterol 1976; 11: 11–15.PubMedGoogle Scholar
  13. 13.
    Yanatori Y, Fujita T. Hypertrophy and hyperplasia in the endocrine and exocrine pancreas of rats fed soybean trypsin inhibitor or repeatability injected with pancreozymin. Arch Histol Jap 1976; 39: 67–78.PubMedGoogle Scholar
  14. 14.
    Fujita T, Matsunari Y, Sato I, Hayashi M, Koga Y. Effects of oral administration of trypsin inhibitor and repeated injections of pancreozymin on the insulin and glucagon contents of rat pancreas. Endocrinol Japon 1979; 26: 35–39.Google Scholar
  15. 15.
    Ihse I, Lundquist I, Arnesjo B. Oral trypsin-inhibitor-induced improvement of the exocrine and endocrine pancreatic functions in alloxan diabetic rats. Scand J Gastroenterol 1976; 11: 363–368.PubMedGoogle Scholar
  16. 16.
    Tamura Y, Hirado M, Okamura K, Minato Y Fujii S. Synthetic inhibitors of trypsin, plasmin, kallikrein, thrombin, Cir, and C1 esterase. Biochim Biophys Acta 1977; 484: 417–422.PubMedGoogle Scholar
  17. 17.
    Scow RO, “Total” pancreatectomy in the rat; operation, effects, and postoperative care. Endocrinology 1957; 60: 359–367.PubMedCrossRefGoogle Scholar
  18. 18.
    Imondi AR, Stradley RO, Wolgemuth R. Synthetic peptides in the diagnosis of exocrine pancreatic insufficiency in animals. Gut 1972; 13: 726–731.PubMedGoogle Scholar
  19. 19.
    Gry K, Wolf RH, Imondi AR, Felsenfeld O. Exocrine pancreatic function in protein-deficient patas monkeys studied by means of a test meal and an indirect pancreatic function test. Gastroenterology 1975; 68: 488–494.Google Scholar
  20. 20.
    Imamura K, Nakamura T, Miyazawa T, Abe Y, Kobayashi M, Takebe K. Oral administration of chymotrypsin labile peptide for a new test of exocrine pancreatic function (PFT) in comparison with pancreozymin-secretin test. Am J Gastroenterol 1978; 69: 572–578.PubMedGoogle Scholar
  21. 21.
    Hyvarien A, Nikkila EA. Specific determination of blood glucose with o-toluidine. Clin Chim Acta 1962; 7: 140–143.CrossRefGoogle Scholar
  22. 22.
    Lundbaek K: Intravenous glucose tolerance as a tool in definition and diagnosis of diabetes mellitus. Brit Med J 1962; 1: 1507–1513.PubMedCrossRefGoogle Scholar
  23. 23.
    Morishita K, Kuroda K, Ichihara K, Tarui S, Shima K. Radioimmunoassay of insulin with the use of Sephadex-coupled antibody. Modern Med 1972; 27: 1037–1042.Google Scholar
  24. 24.
    Yamato C, Kinoshita K. A simple assay for measurement of urinary p-aminobenzoic acid in the oral pancreatic function test. Anal Biochem 1979; 98: 13–17.PubMedCrossRefGoogle Scholar
  25. 25.
    Lundquist I. Acid amyloglucosidase and carbohydrate regulation. I. Effect of exogenous amyloglucosidase on tissue glycogen, blood glucose and plasma insulin. Horm Metab Res 1972; 4: 151–158.PubMedGoogle Scholar
  26. 26.
    Guilbault GG, Rietz EB. Enzymatic, fluorometric assay of amylase in serum. Clin Chem 1976; 22: 1702–1704.PubMedGoogle Scholar
  27. 27.
    Vogel WC, Zieve L. A rapid and sensitive turbidimetric method for serum lipase based upon differences between the lipases of normal and pancreatitis serum. Clin Chem 1963; 9: 168–181.PubMedGoogle Scholar
  28. 28.
    Bossak HN, Rosenberg AA, Harris AD. A quantitative turbidimetric method for the determination of spinal fluid protein. J Ven Dis Inform 1949; 30: 100–103.Google Scholar
  29. 29.
    Sandmeyer W. Üeber die folgen der partiellen Pankreasexstirpartion beim Hund. Z Biol 1895; 31: 12–85.Google Scholar
  30. 30.
    Yasugi H, Mizumoto R, Sakurai H, Honjo I. Changes in carbohydrate metabolism and endocrine function of remnant pancreas after major pancreatic resection. Am J Surg 1976; 132: 577–580.PubMedCrossRefGoogle Scholar
  31. 31.
    Higuchi A, Yasugi H, Yokota T, Tobe T, Mizumoto R. Changes of pancreatic exocrine function after major resection of the pancreas in dogs. Gastroenterol Japan 1979; 14: 316–326.Google Scholar
  32. 32.
    Martin JM, Lacy PR. The prediabetic period in partially pancreatectomized rats. Diabetes 1963; 12: 238–243.Google Scholar
  33. 33.
    Yonemura Y: The endocrine function of the pancreas after partial pancreatectomy-with special reference to the resected size of the pancreas. J Juzen Med Sci 1979; 88: 307–337.Google Scholar
  34. 34.
    Hotz J, Goberna R, Clodi PH. Reserve capacity of the exocrine pancreas. Digestion 1973; 9: 212–223.PubMedCrossRefGoogle Scholar
  35. 35.
    Melmed RN, Turner RC, Holt SJ. Intermediate cells of the pancreas. II. The effects of dietary soybean trypsin inhibitor on acinar-cell structure and function in the rat. J Cell Sci 1973; 13: 279–295.PubMedGoogle Scholar
  36. 36.
    Hagino S. The relationship between endocrine and exocrine pancreatic functions after pancreatectomy in rats. J Juzen Med Sci 1980; 89: 433–452.Google Scholar
  37. 37.
    Soling HD, Unger KO. The role of insulin in the regulation of α-amylase synthesis in the rat pancreas. Europ J Clin Invest 1972; 2: 199–212.PubMedCrossRefGoogle Scholar
  38. 38.
    Lehv M, Fitzgerald PJ. Pancreatic acinar cell regeneration. Am J Pathol 1968; 53: 514–535.Google Scholar
  39. 39.
    Pearson KW, Scott D, Torrance B. Effects of partial surgical pancreatectomy in rats. Gastroenterology 1977; 72: 469–473.PubMedGoogle Scholar
  40. 40.
    Fölsch UR, Wormsley KG. The pancreatic secretion of enzymes in rats. Scand J Gastroenterol 1974; 9: 679–683.PubMedGoogle Scholar

Copyright information

© The Japan Surgical Society 1984

Authors and Affiliations

  • Nobuo Baba
    • 1
  • Takashi Suzuki
    • 1
  • Takayoshi Tobe
    • 1
  1. 1.The First Department of Surgery, Faculty of MedicineKyoto UniversityKyotoJapan

Personalised recommendations