Abstract
For use in routine clinical studies, modifications in Hamburger's stem cell assay were made. A multiplate with 24 wells made feasible, handling of a large number of samples. The addition of anticancer drugs to the bottom layer of agar led to avoidance of exposure to drugs before cell plating and evaluation of the effect of long-acting drugs such as 5-fluorouracil. Storage of the test plate including anticancer drugs in a freezer at −70°C showed no loss of the colony forming activity. This method is simple, reliable and readily facilitated.
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References
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Ichihashi, H., Sakoh, T. & Kondo, T. In vitro soft agar assay by use of multiplate for screening of tumor sensitivity to anticancer drugs. The Japanese Journal of Surgery 13, 549–553 (1983). https://doi.org/10.1007/BF02469503
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DOI: https://doi.org/10.1007/BF02469503