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Analysis of active ingredients and preservatives in creams by capillary electrochromatography (CEC)

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Summary

Capillary electrochromatography (CEC) is an analytical method of growing interest to the pharmaceutical industry. Because CEC is a combination of high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) it has the advantages of the high selectivity of HPLC and the high efficiency of CE. The capillaries can be packed with different packing materials. The mixed-mode phase consisting of a strong cation exchanger and alkyl chains on the silica surface has some advantages over conventional reversed-phase (RP) packing materials. In this article the authors describe the use of CEC to separate the active ingredients and preservatives in creams from different manufactures. On the basis of the high efficiency of CEC it was possible to develop one isocratic method for all the compounds in the different samples. The method was validated with regard to identification, reproducibility, linearity, limit of detection, and quantitation.

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References

  1. V. Pretorius, B. J. Hopkins, J. D. Schiecke, J. Chromatogr.99, 23 (1974).

    Article  CAS  Google Scholar 

  2. J. W. Jorgenson, K. D. Lukacs, Anal. Chem.53, 1298 (1981).

    CAS  Google Scholar 

  3. J. W. Jorgenson, K. D. Lukacs, J. Chromatogr.218, 209 (1981).

    Article  CAS  Google Scholar 

  4. J. Knox, I. Grant, Chromatographia24, 135 (1987).

    CAS  Google Scholar 

  5. J. Knox, Chromatographia26, 329 (1988).

    Article  CAS  Google Scholar 

  6. J. Knox, I. Grant, Chromatographia32(7/8), 317 (1991).

    Article  CAS  Google Scholar 

  7. J. Knox, J. Chromatogr. A680, 3 (1994).

    Article  CAS  Google Scholar 

  8. G. Choudhary, Cs. Horváth, J. Chromatogr. A781, 161 (1997).

    Article  CAS  Google Scholar 

  9. A. S. Rathore, Cs. Horváth, J. Chromatogr. A781, 185 (1997).

    Article  CAS  Google Scholar 

  10. S. Lüdtke, T. Adam, K. K. Unger, J. Chromatogr. A786, 229 (1997).

    Article  Google Scholar 

  11. R. E. Majors, LC-GC16(2), 96 (1998).

    CAS  Google Scholar 

  12. J. H. Miyawa, D. K. Lloyd, M. S. Alasandro, J. High Resol. Chromatogr.21, 161 (1998).

    Article  CAS  Google Scholar 

  13. N. W. Smith, M. B. Evans, Chromatographia41(3/4), 197 (1995).

    Article  CAS  Google Scholar 

  14. M. M. Robinson, M. G. Gikalo, P. Myers, M. E. Euerby, K. D. Bartle, J. Microcol. Sep.9(5) 357 (1997).

    Article  Google Scholar 

  15. M. R. Euerby, D. Gilligan, C. M. Johnson, S. C. P. Roulin, P. Myers, K. D. Bartle, J. Microcol. Sep.9(5), 373 (1997).

    Article  CAS  Google Scholar 

  16. R. J. Boughtflower, T. Underwood, C. J. Paterson, Chromatographia40, 329 (1995).

    Article  CAS  Google Scholar 

  17. R. Boughtflower, T. Underwood, J. Maddin, Chromatographia41(7/8), 398 (1995).

    Article  CAS  Google Scholar 

  18. B. Behnke, E. Grom, E. Bayer, J. Chromatogr. A716, 207 (1995).

    Article  CAS  Google Scholar 

  19. M. Dittmann, G. Rozing, J. Chromatogr. A744, 63 (1996).

    Article  CAS  Google Scholar 

  20. N. W. Smith, M. B. Evans, Chromatographia41(3/4), 197 (1995).

    Article  CAS  Google Scholar 

  21. K. D. Altria, N. W. Smith, C. H. Turnbull, Chromatographia46(11/12), 664 (1997).

    CAS  Google Scholar 

  22. N. C. Gillott, D. A. Barrett, S. P. Nicholas, M. R. Euerby, C. M. Johnson, Anal. Commun.35(7), 217 (1997).

    Article  Google Scholar 

  23. M. R. Euerby, C. M. Johnson, K. D. Bartle, LC-GC16(4), 386 (1998).

    CAS  Google Scholar 

  24. R. H. Asiaie, X. Huang, D. Farnan, C. Horvath, J. Chromatogr. A806(2), 251 (1998).

    Article  CAS  Google Scholar 

  25. Hewlett Packard publication 5965-9028E.

  26. Thomas Adam, Thesis, University of Mainz, Germany (1998).

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Author notes

  1. Th. Adam

    Present address: Bayer AG, PH-OP-ELB-QW, Gebäude 302, 42096, Wuppertal

Authors and Affiliations

  1. Pharmaceutical and Analytical Development, Novartis Pharma AG, 4002, Basel, Switzerland

    Th. Adam & M. Krämer

Authors
  1. Th. Adam
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  2. M. Krämer
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Adam, T., Krämer, M. Analysis of active ingredients and preservatives in creams by capillary electrochromatography (CEC). Chromatographia 49 (Suppl 1), S35–S40 (1999). https://doi.org/10.1007/BF02468974

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  • DOI: https://doi.org/10.1007/BF02468974

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