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The correlation between humoral antibodies and rejection after renal transplantation

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Abstract

Sera from 36 renal transplant patients were selected at random and screened for cytotoxic antibodies to a panel of 50 normal lymphocytes and 3 lymphoblasts. 11 of these patients had received more than one kidney transplant. None of the primary renal transplants demonstrated cytotoxic antibodies to the panel of normal cells. However, 4 of the retransplant patients were found to possess cytoxins. These 4 sera were cytotoxic to the normal cell panel (10%, 20%, 50% and 70% of panel lysed) as well as to certain lymphoblasts. 30% of the sera negative to the normal cell panel demonstrated cytoxins to the lymphoblasts; 15% positive to only the B-lymphoblasts, 5% positive to only the T-lymphoblasts and 10% showed cytotoxic antibodies to both T and B-lymphoblasts and 10% showed cytotoxic antibodies to both T and B-lymphoblasts.

No correlation between cytotoxicity and inhibition of MLR was obvious. Of the 14 sera showing cytotoxicity to lymphoblasts, 9 exhibited MLR inhibition. However, inhibition of MLR was not found to be restricted to B-cell cytotoxic sera.

These sera were tested by radioimmunoassay. A good correlation was found between acute rejection and percent increase in direct binding of patients sera to radiolabelled B-lymphoblast membranes. Of the 36 sera tested the six sera obtained from patients in acute rejection bound the iodinated lysate to a significantly greater amount than the controls.

As yet, no characterization of the antigen specificities has been found.

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Authors and Affiliations

  1. Department of Surgery, Kyoto National Hospital, Kyoto, Japan

    Hidenori Yoshioka

  2. University of Colorado Medical Center, Colorado, USA

    Robert T. McCalmon Jr.

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  1. Hidenori Yoshioka
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  2. Robert T. McCalmon Jr.
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Yoshioka, H., McCalmon, R.T. The correlation between humoral antibodies and rejection after renal transplantation. The Japanese Journal of Surgery 9, 95–101 (1979). https://doi.org/10.1007/BF02468843

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  • DOI: https://doi.org/10.1007/BF02468843

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