Chromatographia

, Volume 49, Issue 9–10, pp 552–556 | Cite as

Separation ofPodophyllum lignans by micellar electrokinetic capillary chromatography (MECC)

  • M. Ganzera
  • R. M. Moraes
  • I. A. Khan
Originals

Summary

Separation of the mainPodophyllum lignans by MECC has been achieved using a phosphate-borate buffer system with the pH of 7.25, containing 25 mM SDS and 15% methanol. Optimum temperature and voltage were found to be 35°C and 20kV, respectively, UV-detection was performed at 220 nm. The method was successfully used to analyse different samples ofPodophyllum peltatum collected in the southern USA, and showed great qualitative and quantitative variations among these populations. The quantitative MECC results for podophyllotoxin were in good agreement to those obtained by HPLC.

Key Words

Micellar electrokinetic chromatography Lignans Podophyllum peltatum 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1999

Authors and Affiliations

  • M. Ganzera
    • 1
  • R. M. Moraes
    • 1
  • I. A. Khan
    • 1
    • 2
  1. 1.National Center for the Development of Natural Products, Research Institute of Pharmaceutical Sciences, School of PharmacyThe University of MississippiUniversityUSA
  2. 2.Department of Pharmacognosy, School of PharmacyThe University of MississippiUniversityUSA

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