Separation ofPodophyllum lignans by micellar electrokinetic capillary chromatography (MECC)
Separation of the mainPodophyllum lignans by MECC has been achieved using a phosphate-borate buffer system with the pH of 7.25, containing 25 mM SDS and 15% methanol. Optimum temperature and voltage were found to be 35°C and 20kV, respectively, UV-detection was performed at 220 nm. The method was successfully used to analyse different samples ofPodophyllum peltatum collected in the southern USA, and showed great qualitative and quantitative variations among these populations. The quantitative MECC results for podophyllotoxin were in good agreement to those obtained by HPLC.
Key WordsMicellar electrokinetic chromatography Lignans Podophyllum peltatum
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- H. F. Stählin, A. Wartburg Cancer Res.51, 5 (1991).Google Scholar
- S. Forster, Acta Hort.330, 67 (1993).Google Scholar
- W. Meijer, Econ Bot.28, 68 (1974).Google Scholar
- A. Y. Leung, Encyclopedia of Common Natural Ingredie Used in Food, Drugs and Cosmetics, John Wiley & Sons, New York, 1980, p. 270.Google Scholar
- G. A. Kuznetsova, V. P. Bogdanova, Rast. Resur.6, 240 (1970).Google Scholar
- J. Bastos, W. Kopycki, C. Burandt, D. Nanayakkara, J. McChesney, Phytochem. Anal.6, 101 (1995).Google Scholar
- D. Heiger, High Performance Capillary Electrophoresis, Hewlett-Packard Company, Waldbronn, Germany, 1992, p. 58.Google Scholar