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The effect of conductivity tuning in chiral separations by CE; Using hydroxypropyl-β-cyclodextrin in combination with tetraalkylammonium ions

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Summary

Different approaches how to handle the electromigration dispersion process that occurs in separation and determination of enantiomers are presented. The use of cyclodextrins as chiral selectors in resolution enantiomers involves the possibility to tune the conductivity of the sample band in order to obtain symmetrical and efficient peaks. Determination of impurities that migrate in the rear part of an overloaded main peak can be accomplished if the conductivity of the background electrolyte (BGE) is adapted to the conductivity of the sample band. This strategy was shown in determination of the content of D-sotalol in a mixture of L and D-sotalol. The efficiency and the symmetry of the overloaded L-sotalol peak was substantially improved by substitution of tetrabutylammonium ions for tetrapentylammonium ions as co-ions in the BGE. In this system it was possible to determine 0.2% w/w of the chiral impurity D-sotalol. A resolution model is presented and used qualitatively in the study where the complexation between the tetraalkyllammonium ions and the cyclodextrins is taken into account.

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Authors and Affiliations

  1. dept. HQA, Astra Production Chemicals AB, 151 85, Södertälje, Sweden

    O. Stålberg

  2. Analytical Pharmaceutical Chemistry Dept., Biomedical Center, University of Uppsala, P.O. Box 574, 751 23, Uppsala, Sweden

    M. Hedeland, C. Pettersson & D. Westerlund

Authors
  1. O. Stålberg
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  2. M. Hedeland
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  3. C. Pettersson
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  4. D. Westerlund
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Stålberg, O., Hedeland, M., Pettersson, C. et al. The effect of conductivity tuning in chiral separations by CE; Using hydroxypropyl-β-cyclodextrin in combination with tetraalkylammonium ions. Chromatographia 48, 415–421 (1998). https://doi.org/10.1007/BF02467713

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  • DOI: https://doi.org/10.1007/BF02467713

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