, Volume 48, Issue 3–4, pp 190–196 | Cite as

Dye-coated stationary-phases: A retention model for anions in ion-interaction chromatography

  • D. Tonelli
  • S. Zappoli
  • B. Ballarin


The separation of inorganic anions (NO3 , NO2 , Cl, Br, I, SO4 2−, S2O3 2−) by ion-interaction chromatography mediated with a specific dye has been investigated. Chromatography was performed on a LiChrospher RP-18 colum dynamically coated with crystal violet, using acetonitrile-water buffered with phthalate as the mobile phase. The presence of the dye in the eluent enabled indirect spectrophotometric detection of the analytes, which have no significant UV absorption. Retention data were collected for the different anions by varying the composition of the mobile phase according to a full factorial experimental design. A theoretical model for the retention of singly- and doubly-charged analytes, on the basis of the two main processes of ion-exchange and ion-pair formation, has been proposed and validated with the experimental data.

Key Words

Column liquid chromatography Ion-interaction chromatography Dye-coated reversed phase Retention model Experimental design 


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  1. [1]
    H. Small, T. S. Stevens, W. C. Bauman, Anal Chem.47, 1801 (1975).CrossRefGoogle Scholar
  2. [2]
    R. Gloor, E. L. Johnson, J. Chromatogr. Sci.15, 413 (1977).Google Scholar
  3. [3]
    C. Horvath, W. Melander, I. Molnar, P. Molnar, Anal. Chem.49, 2295 (1977).CrossRefGoogle Scholar
  4. [4]
    Z. Iskandarani, D. J. Pietrzyk, Anal. Chem.54, 1065 (1982).Google Scholar
  5. [5]
    G. Schmuckler, B. Rössner, G. Schwedt, J. Chromatogr.302, 15 (1984).CrossRefGoogle Scholar
  6. [6]
    J. J. Stranahan, S. N. Deming, Anal. Chem.54, 2251 (1982).CrossRefGoogle Scholar
  7. [7]
    Y. K. Zhang, H. F. Zou, M. F. Hong, P. C. Lu, Chromatographia32, 538 (1991).CrossRefGoogle Scholar
  8. [8]
    H. Liu, F. F. Cantwell, Anal. Chem.63, 993 (1991).CrossRefGoogle Scholar
  9. [9]
    Q. Xianren, W. Baeyens, J. Chromatogr.456, 267 (1988).CrossRefGoogle Scholar
  10. [10]
    J. G. Chen, S. G. Weber, L. L. Glavina, F. F. Cantwell J. Chromatogr.656, 549 (1992).CrossRefGoogle Scholar
  11. [11]
    R. M. Cassidy, S. Elchuk, Anal. Chem.54, 1558 (1982).CrossRefGoogle Scholar
  12. [12]
    K. Ito, E. Shoto, H. Sunahara, J. Chromatogr.549, 265 (1991).CrossRefGoogle Scholar
  13. [13]
    B. A. Bidlingmeyer, F. V. Warren Jr, Anal. Chem.54, 2351 (1982).CrossRefGoogle Scholar
  14. [14]
    J. Crommen, G. Schill, D. Westerlund, L. Hackzell, Chromatographia24, 252 (1987).Google Scholar
  15. [15]
    M. Eslami, P. Hashemi, M. N. Sarbolouki, J. Chromatogr. Sci.31, 480 (1993) and references cited therein.Google Scholar
  16. [16]
    R. Golombek, G. Schwedt, J. Chromatogr.452, 283 (1988).CrossRefGoogle Scholar
  17. [17]
    T. A. Walker, J. Chromatogr.602, 97 (1992).CrossRefGoogle Scholar
  18. [18]
    J. C. Giddings, Dynamics of Chromatography. Part I, Principles and Theory, Marcel Dekker, New York, 1965, p. 6.Google Scholar
  19. [19]
    G. N. Lewis, T. T. Magel, D. Lipkin, J. Am. Chem. Soc.64, 1774 (1942).CrossRefGoogle Scholar
  20. [20]
    S. N. Deming, S. L. Morgan, Experimental Design: A Chemometric Approach, Elsevier, New York, 1987.Google Scholar
  21. [21]
    A. M. Krstulovic, H. Colin, G. Guiochon, Anal. Chem.54, 2438 (1982).CrossRefGoogle Scholar
  22. [22]
    J. J. Stranahan, S. N. Deming, Anal. Chem.54, 1540 (1982).CrossRefGoogle Scholar
  23. [23]
    E. Arvidsson, L. Hackzell, G. Schill, D. Westerlund, Chromatographia25, 430 (1988).CrossRefGoogle Scholar
  24. [24]
    N. Dyson, Chromatographic Integration Methods, RSC Chromatography Monographs Series, (Series Editor R. M. Smith), Royal Society of Chemistry, Cambridge, 1990.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1998

Authors and Affiliations

  • D. Tonelli
    • 1
  • S. Zappoli
    • 1
  • B. Ballarin
    • 1
  1. 1.Dipartimento di Chimica Fisica ed InorganicaUniversità di BolognaBolognaItaly

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