Summary
Analytical HPLC methods using derivatized amylose chiral stationary phases were developed for the resolution of enantiomers of methoxy an ethyl tetrahydronaphthalenic derivatives, new agonist and antagonist ligands for melatonin receptors. Separation was by normal phase methodology with a mobile phase ofn-hexane-alcohol (methanol, ethanol, 1-propanol or 2-propanol) in various proportions, and a silica-based, amylose tris-(S)-1-phenylethylcarbamate (Chiralpak AS), or tris-3,5-dimethylphenylcarbamate (Chiralpak AD). The mobile phase and the chiral stationary phase were optimized for best resolution. The effects of concentration of alcohol, various aliphatic alcohols in the mobile phase were studied. The effects of substitution were analysed. Baseline separation (Rs>1.5) was easily obtained in man cases.
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Vaccher, C., Fourmaintraux, E., Belloli, E. et al. Enantiomeric resolution of melatonin ligands receptors by liquid chromatography on amylose chiral stationary phases. Chromatographia 48, 790–796 (1998). https://doi.org/10.1007/BF02467649
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DOI: https://doi.org/10.1007/BF02467649