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Chromatographia

, Volume 49, Issue 7–8, pp 374–378 | Cite as

High performance liquid chromatography coupled to nuclear magnetic resonance spectroscopy and mass spectrometry applied to plant products: Identification of ecdysteroids fromSilene otites

  • I. D. Wilson
  • E. D. Morgan
  • R. Lafont
  • J. P. Shockcor
  • J. C. Lindon
  • J. K. Nicholson
  • B. Wright
Originals

Summary

HPLC coupled in parallel to nuclear magnetic resonance (NMR) and mass spectrometry (MS) has been used to obtain1H NMR and mass spectra of a number of ecdysteroids present in an extract of the plantSilene otites. Reversed phase gradient chromatography was performed using a D20-acetonitrile-based solvent system. NMR and mass spectra were obtained for integristerone A, 20-hydroxyecdysone, 2-deoxy-20-hydroxyecdysone and 2-deoxyecdysone to provide structural confirmation using continuous and stopped flow HPLC-NMR. The combined HPLC-NMR-MS system described here provided a more comprehensive analysis of the ecdysteroids present in the extract than HPLC-NMR alone.

Key Words

Column liquid chromatography NMR spectroscopy and mass spectrometry Plant products Silene otites 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1999

Authors and Affiliations

  • I. D. Wilson
    • 1
    • 5
  • E. D. Morgan
    • 1
  • R. Lafont
    • 2
  • J. P. Shockcor
    • 3
  • J. C. Lindon
    • 4
  • J. K. Nicholson
    • 4
  • B. Wright
    • 5
  1. 1.Department of ChemistryUniversity of KeeleKeeleUK
  2. 2.Ecole Normale Superieure, Departement de BiologieCNRS EP 119Paris Cedex O5France
  3. 3.Stine-Haskell Research CenterDupont PharmaceuticalsNewarkUSA
  4. 4.Biological Chemistry, Biomedical Sciences Division, Imperial College of Science, Technology and MedicineUniversity of London, South KensingtonLondonUK
  5. 5.Zeneca PharmaceuticalsMacclesfieldUK

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