Summary
Peak compression of a dihydropyridine drug, clevidipine, is obtained in both analytical and semi-preparative scale supercritical fluid chromatography, resulting in extremely high apparent efficiencies. The observed effect, when utilising a carbon dioxide/2-propanol mobile phase with a bare silica stationary phase, is achieved when the retention of the clevidipine peak is controlled to coalesce with a system peak, generated as a result of having water in the sample. Apparent efficiencies of 350,000 and 170,000 plates meter−1 were obtained when 0.25 and 0.5 mg, respectively, are directly injected to a 200×4.6 mm ID 5 μm Hypersil silica packed column. The effect was extended to a semi-preparative system where apparent efficiencies in the region of 2,000,000 plates meter−1 were observed when 0.3 mg of a clevidipine sample containing 80% water was injected to a 250×10 mm I.D. column containing 5-μm Hypersil silica particles.
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References
T. A. Berger in “Packed Column SFC” Roger M. Smith, Ed., RCS Chromatography Monographs, The Royal Society of Chemistry, Cambridge, UK, 1996.
A. Salvador, M. A. Jaime, G. Becerra, M. De La Guardia, Fres. J. Anal. Chem.356, 109 (1996).
T. A. Berger, W. H. Wilson, J. Pharm. Sci.83, 281 (1994).
T. A. Berger, W. H. Wilson, J. Pharm. Sci.83, 287 (1994).
J. T. Strode III, L. T. Taylor, A. L. Howard, D. P. Ip, M. A. Brooks, J. Pharm. Biomed. Anal.12, 1003 (1994).
L. Karlsson, O. Gyllenhaal, A. Karlsson, J. Gottfries, J. Chromatogr. A749, 193 (1996).
O. Gyllenhaal, A. Karlsson, J. Vessman, in “Supercritical Fluid Chromatography with packed columns”, K. Anton, C. Berger, Ed., Marcel Dekker, Inc., New York, USA, 1998, p. 273.
O. Gyllenhaal, J. Vessman, J Chromatogr.,628, 275 (1993).
K. L. Williams, L. C. Sander, J. Chromatogr. A785, 149 (1997).
R. M. Smith, in “Supercritical Fluid Chromatography with packed columns”, K. Anton, C. Berger, Ed., Marcel Dekker, Inc., New York, USA, 1998, p. 223.
C. Wolf, W. H. Pirkle, in “Supercritical Fluid Chromatography with packed columns”, K. Anton, C. Berger, Ed., Marcel Dekker, Inc., New York, USA, 1998, p. 251.
W. F. Kline, B. K. Matuszewski, The application of packed column supercritical fluid chromatography for chiral separation of selected drug candidates. Poster presentation at the 8th International Symposium on Supercritical Fluid Chromatography and Extraction, St. Louis, USA, July 12–16, 1998.
J. Y. Gao, C. Kibbey, Characterisation of combinatorial libraries by supercritical fluid chroma, K. Anton, C. Berger, Ed., Marcel Dekker, Inc., New York, USA, 1998, p.59.
T. A. Berger, SFC for high throughput screening in drug discovery. Presentation at the 8th International Symposium on Supercritical Fluid Chromatography and Extraction, St. Louis, USA, July 12–16, 1998.
C. Berger, M. Perrut, J. Chromatogr.505, 37 (1990).
F. Vérillon, K. Coleman, in “Supercritical Fluid Chromatography with packed columns”, K. Anton, C. Berger, Ed., Marcel Dekker, Inc., New York, USA, 1998, p. 59.
P. Jusforgues, M Shaimi, D Barth, in “Supercritical Fluid Chromatography with packed columns”, K. Anton, C. Berger, Ed. Marcel Dekker, Inc. New York, USA, 1998, p. 403.
K. D. Bartle, C. D. Bevan, A. A. Clifford, S. A. Jafar, N. Malak, M. S. Verrall, J. Chromatogr. A697, 579 (1995).
D. Carlsson, J. T. Strode III, O. Gyllenhaal, A. Karlsson, L. Karlsson, Chromatographia44, 289 (1997).
J. T. Strode III, O. Gyllenhaal, A. Torstensson, A. Karlsson, L. Karlsson, J. Chrom. Sci.36, 257 (1998).
J. A. Graham, L. B. Rogers, J. Chrom. Sci.18, 75 (1980).
R. D. Smith, E. G. Chapman, B. W. Wright, Anal. Chem.57, 2829 (1985).
A. Wilsch, G. M. Schneider, J. Chromatogr. A357, 239 (1986).
M. R. Euerby, D. Gilligan, C. M. Johnson, S. C. P. Roulin, P. Myers, K. D. Bartle, J. Microcol. Sep.9, 373 (1997).
T. Fornstedt, D. Westerlund, A. Sokolowski, J. Liq. Chrom.11, 2645 (1988).
T. Fornstedt, D. Westerlund, A. Sokolowski, J. Chromatogr.506, 61 (1990).
T. Fornstedt, D. Westerlund, A. Sokolowski, J. Chromatogr.535, 93 (1990).
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Owens, P.K., Gyllenhaal, O., Karlsson, A. et al. Peak compression in semi-preparative supercritical fluid chromatography. Chromatographia 49, 327–332 (1999). https://doi.org/10.1007/BF02467565
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DOI: https://doi.org/10.1007/BF02467565