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FMOC-Cl as derivatizing agent for the analysis of amino acids and dipeptides by the absolute analysis method

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Summary

One classical method for quantitation of amino acids in proteins is hydrolysis of the proteins and determination of the free amino acids. Although the drastic experimental conditions necessary for complete hydrolysis always cause degradation of some of the amino acids, if mild hydrolysis conditions are used, a mixture of amino acids and oligopeptides is obtained. If these conditions are adequately tuned, the oligopeptides are almost exclusively dipeptides. For this reason we have initiated a study to find a derivatizing agent suitable for the analysis of amino acids and dipeptides by an absolute method of quantitation already tested for amino acids. FMOC-Cl was found to be a suitable derivatizing agent for this purpose.

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References

  1. D. Roach, C. W. Gehrke, J. Chromatogr.52, 393 (1970).

    Article  CAS  Google Scholar 

  2. G. J. Schmidt, D. C. Olson, W. Slavin, J. Liq. Chromatogr.2, 1031 (1979).

    CAS  Google Scholar 

  3. B. N. Jones, P. Paabo, S. Stein, J. Liq. Chromatogr.4, 565 (1981).

    CAS  Google Scholar 

  4. S. Einarsson, B. Josefsson, S. Lagerkvist, J. Chromatogr.282, 609 (1983).

    Article  CAS  Google Scholar 

  5. U. Bütikofer, D. Fuchs, J. O. Bosset, W. Gmür, Chromatographia31, 441 (1991).

    Article  Google Scholar 

  6. J. R. E. Lewis, J. S. Morley, R. F. Venn, J. Chromatogr.615, 37 (1993).

    CAS  Google Scholar 

  7. A. G. Schepky, K. W. Bensch, P. Schulz-Knappe, W.-G. Forssmann, Biomed. Chromatogr.8, 90 (1994).

    Article  CAS  Google Scholar 

  8. J. Kirschbaum, B. Luckas, W. D. Beinert, Int. Lab. July/August, 27 (1994).

    Google Scholar 

  9. J. M. Roturier, D. Le Bars, J. C. Gripon, J. Chromatogr. A696, 209 (1995).

    Article  CAS  Google Scholar 

  10. J. K. Lim, C. H. Wang, Clin. Chem.26, 579 (1980).

    Google Scholar 

  11. R. L. Heinrikson, S. C. Meredith, Anal. Biochem.136, 65 (1984).

    Article  CAS  Google Scholar 

  12. G. Torsi, G. Chiavari, C. Laghi, A. M. Asmundottir, F. Fagioli, R. Vecchietti, J. Chromatogr.482, 207 (1989).

    Article  CAS  Google Scholar 

  13. G. Torsi, G. Chiavari, C. Laghi, A. M. Asmundottir, J. Chromatogr.518, 135 (1990).

    Article  CAS  Google Scholar 

  14. G. Torsi, G. Chiavari, M. T. Lippolis, LC-GC Int.1(5), 37 (1991).

    Google Scholar 

  15. G. Torsi, A. M. Gioacchini, N. Gandini, Annali di Chimica83, 345 (1993).

    CAS  Google Scholar 

  16. B. Gustavsson, I. Betner, J. Chromatogr.507, 67 (1990).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University of Ferrara, via L. Borsari, 46, 44100, Ferrara, Italy

    D. Melucci

  2. Analytical and Testing Center, Beijing Normal University, 100875, Beijing, P. R. China

    M. Xie

  3. Department of Chemistry ‘G. Ciamician’, University of Bologna, via F. Selmi, 2, 40126, Bologna, Italy

    P. Reschiglian & G. Torsi

Authors
  1. D. Melucci
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  2. M. Xie
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  3. P. Reschiglian
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  4. G. Torsi
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Melucci, D., Xie, M., Reschiglian, P. et al. FMOC-Cl as derivatizing agent for the analysis of amino acids and dipeptides by the absolute analysis method. Chromatographia 49, 317–320 (1999). https://doi.org/10.1007/BF02467563

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  • DOI: https://doi.org/10.1007/BF02467563

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