Summary
A simple, economical and efficient HPLC method has been developed for the separation and determination of the individual glucosinolates in rapessed and mustard. The method involves single-step extraction of glucosinolates with boiling water and separation of the individual glucosinolates on a Novapack RP-18 column (3.9 mm ×150mm) with 0.2 M ammonium sulphate as mobile phase. Peaks were monitored at 229 nm. All major glucosinolates could be eluted within 10 min. The method proved effective for routine analysis of glucosinolates.
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Kaushik, N., Agnihotri, A. High-performance liquid chromatographic method for separation and quantification of intact glucosinolates. Chromatographia 49, 281–284 (1999). https://doi.org/10.1007/BF02467557
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DOI: https://doi.org/10.1007/BF02467557