Summary
Phenolic acids fromLycopus europaeus L. (Lamiaceae) were chromatographed on thin layers of precoated silica, LiChrospher silica, propylamine, diol layers using stepwise multiple development and, additionally, on an octadecyl layer with an isocratic mobile phase. The eluent contained decreasing concentrations of formic acid in a mixture of diisopropyl ether-cyclohexane for silica gel plates or methyl-ethylketone inn-hexane for diol plates and increasing concentrations of acetic acid in acetone for the propylamine layer. Extracts were separated into 25 components, 14 of which were identified using a mixture of standards. 2,4-Dihydroxybenzoic, gentisic, protocatechuic and 3,5-dihydroxybenzoic acids were ascribed to this genus for the first time. The adsorbent-eluent systems described were very efficient and rapid.
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Fecka, I., Cisowski, W. Multiple gradient development TLC in analysis of complex phenolic acids fromLycopus europeaus L.. Chromatographia 49, 256–260 (1999). https://doi.org/10.1007/BF02467553
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DOI: https://doi.org/10.1007/BF02467553