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Separation of catecholamines and serotonin by micellar electrokinetic chromatography with UV detection

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Summary

The possibility of separation of the main catecholamines, serotonin and 3,4-L-dihydroxyphenylalanine by micellar electrokinetic chromatography (MEKC) with UV detection has been investigated using phosphate and phosphate-borate buffers with sodium dodecylsulfate as electrolytes. The results demonstrate the applicability of MEKC-UV detection systems for biomedical analysis of catecholamines and serotonin. Detection limits in the 10−7 mol L−1 range could be achieved.

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Authors and Affiliations

  1. Laboratory of Neurobiology, Tbilisi State University, Tshavtshavadze av, 1, 380028, Tbilisi, Georgia

    N. Shakulashvili

  2. Instrumentelle Analytik/Umweltanalytik, Universität des Saarlandes, Im Stadtwald, 66123, Saarbrücken, Germany

    Ch. Finkler & H. Engelhardt

Authors
  1. N. Shakulashvili
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  2. Ch. Finkler
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  3. H. Engelhardt
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Shakulashvili, N., Finkler, C. & Engelhardt, H. Separation of catecholamines and serotonin by micellar electrokinetic chromatography with UV detection. Chromatographia 47, 89–92 (1998). https://doi.org/10.1007/BF02466792

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  • DOI: https://doi.org/10.1007/BF02466792

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