Summary
A sensitive and selective high-performance liquid chromatographic method has been developed for monitoring clozapine levels in human plasma. Chromatography was performed on a reversed-phase column (C8, 150 mm×4.6 mm i.d., 5 μm) with acetonitrile-aqueous sodium acetate solution, 88∶12 (v/v), as mobile phase; the flow rate was 1 mL min−1. Clozapine oxidation at +800 mV was detected amperometrically. Response was linearly dependent on concentration over the range 50–1500 ng mL−1 clozapine in plasma. Sample preparation by solid-phase extraction before HPLC analysis gave high extraction yield (94%). The accuracy and precision of the method were both very good (recovery: 97%;RSD<3.3%).
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Raggi, M.A., Bugamelli, F., Mandrioli, R. et al. Determination of clozapine in human plasma by solid-phase extraction and liquid chromatography with amperometric detection. Chromatographia 47, 8–12 (1998). https://doi.org/10.1007/BF02466779
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DOI: https://doi.org/10.1007/BF02466779