, Volume 44, Issue 11–12, pp 619–622 | Cite as

HPLC analysis of boldine in pharmaceuticals

  • D. De Orsi
  • L. Gagliardi
  • F. Manna
  • D. Tonelli


A method is proposed for extraction, separation, identification and quantitative measurement of boldine (I) in pharmaceuticals. The extraction of (I) from the matrix (syrups, tablets, extracts) was realized with aqueous ammonium chloride (0.02 M, pH=3.0 with perchloric acid) and the clean-up of the extract was performed by solid-phase extraction by means of cartridges containing a strong cation-exchange sorbent, from which (I) was eluted with aqueous ammonium chloride (1 M, pH=10.0 with ammonia). The final eluate was examined by HPLC. Chromatography was performed on a C-8 column with a gradient elution from 15:85 to 30:70 of acetonitrile-water containing 0.1 M sodium perchlorate (pH=3.0), in 20 min. A diode array detector was used at 302 nm for detection. The method gives recovery values greater than 98% and the reproducibility is within 6.0%.

Key Words

Column liquid chromatography Boldine in pharmaceuticals 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    H. Schindler, Arzneimittel-forsch.7, 747 (1957).Google Scholar
  2. [2]
    Martindale, The Extra Pharmacopoeia, Pharmaceutical Press, London, 28th ed., 1982.Google Scholar
  3. [3]
    K. Genest, D. W. Hughes, Can. J. Pharm. Sci.3, 84 (1968).Google Scholar
  4. [4]
    L. Fleischmann, G. Maffi, P. G. Mezzanzanica, Boll. Chim. Farm.114, 534 (1975)Google Scholar
  5. [5]
    P Pietta, P. Mauri, E. Manera, P. Ceva, J. Chromatogr.457, 442 (1988).CrossRefGoogle Scholar
  6. [6]
    T.J. Betts, J. Chromatogr.511, 373 (1990).CrossRefGoogle Scholar
  7. [7]
    A. Perico, A. Cocchini, R. Noferini, C. Mannucci, A. Cambi, J. Liq. Chromatogr.15, 617 (1992).Google Scholar
  8. [8]
    H. Speisky, B.K. Cassels, S. Nieto, A. Valenzuela, L. J. Nunez-Vergara, J. Chromatogr.612 315 (1993).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1997

Authors and Affiliations

  • D. De Orsi
    • 1
  • L. Gagliardi
    • 1
  • F. Manna
    • 2
  • D. Tonelli
    • 3
  1. 1.Laboratorio di Chimica del FarmacoIstituto Superiore di SanitàRomeItaly
  2. 2.Dipartimento di Chimica e Technologia delle Sostanze Biologicamente AttiveUniversità “La Sapienza”RomeItaly
  3. 3.Dipartimento di Chimica Fisica ed InorganicaUniversità di BolognaBolognaItaly

Personalised recommendations