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Characterization of peak purity by fast multiscan circular dichroism detection

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Summary

A method has been developed to detect inhomogeneity of apparently homogeneous peaks of very similar analytes. The method utilizes the rapid scan feature of state-of-the-art spectrometers/detectors that allow the recording of up to 30 spectra in a single chromatographic peak. Sensitivity and selectivity are enhanced by chiroptical/optical detection. Thus, identification of “front” and “rear” components of the peak can be carried out. The method is exemplified by mixtures of codeine, hydrocodone and oxycodone as analytes.

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Authors and Affiliations

  1. Institute of Pharmaceutical Chemistry, Semmelweis University, Högyes Endre u. 9, 1092, Budapest, Hungary

    P. Horváth, A. Gergeley & B. Noszál

Authors
  1. P. Horváth
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  2. A. Gergeley
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  3. B. Noszál
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Horváth, P., Gergeley, A. & Noszál, B. Characterization of peak purity by fast multiscan circular dichroism detection. Chromatographia 48, 584–588 (1998). https://doi.org/10.1007/BF02466654

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  • DOI: https://doi.org/10.1007/BF02466654

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