Summary
It was surprisingly found in TLC that double spraying of plates could enhance fluorescence of dansyl derivatives 200–360 fold after spraying with 20% triethanolamine in propane-2-of followed by 30% paraffin oil in hexane on a developed plate containing dansyl chloride derivatives. Visual detection limits as low as 1–6 pmole per spot even a month after spraying with a reproducibility of about 5–6% rsd were obtained. Spraying calibration graphs showed a dose-dependency of at least 1 pmole up to 1.5 nmole per spot. The stability of fluorescence of derivatives to light was increased and spectra of compounds acquired a hypochromic shift in which the color changed from yellow to blue. The graphical method employing the “organic concept” is introduced to explain this kind of synergistic effect of double spraying using different solvents.
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Yun, L., Weicheng, Z. Physico-chemical phenomena of double-layer spray and effect on enhancement and stabilization of fluorescence. Chromatographia 48, 548–554 (1998). https://doi.org/10.1007/BF02466648
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DOI: https://doi.org/10.1007/BF02466648