The effects of pH, ionic strength and buffer concentration of mobile phase onR F of acidic compounds in ion-pair TLC
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The effects of some factors important in ion-pair, high-performance liquid chromatography were studied in ion-pair, thin-layer chromatography. Tetramethyl and cetyltrimethylammonium salts were used as ion-pairing reagents. As stationary phases, silica gel and chemically bonded reversed-layers (C18) were used. Layers were impregnated with ion-pairing reagent prior to chromatography. In some cases the stationary phase was treated with buffer at different concentrations. The mobile phase contained methanol and water, in one set of experiments buffer, salt for adjustment of ionic strength and ion-pairing reagent were added. The migration behaviour of different benzoic acids was studied. Several problems of ion-pair thin-layer chromatography are discussed.
Key WordsThin-layer chromatography Ion-pairing reagents pH of stationary phase Benzoic acid
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