Chromatographia

, Volume 48, Issue 7–8, pp 511–516 | Cite as

The effects of pH, ionic strength and buffer concentration of mobile phase onR F of acidic compounds in ion-pair TLC

  • M. Lukács
  • G. Barcsa
  • K. Kovács-Hadady
Originals

Summary

The effects of some factors important in ion-pair, high-performance liquid chromatography were studied in ion-pair, thin-layer chromatography. Tetramethyl and cetyltrimethylammonium salts were used as ion-pairing reagents. As stationary phases, silica gel and chemically bonded reversed-layers (C18) were used. Layers were impregnated with ion-pairing reagent prior to chromatography. In some cases the stationary phase was treated with buffer at different concentrations. The mobile phase contained methanol and water, in one set of experiments buffer, salt for adjustment of ionic strength and ion-pairing reagent were added. The migration behaviour of different benzoic acids was studied. Several problems of ion-pair thin-layer chromatography are discussed.

Key Words

Thin-layer chromatography Ion-pairing reagents pH of stationary phase Benzoic acid 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    S. Lewis, I. D. Wilson, J. Chromatogr.312, 133 (1984).CrossRefGoogle Scholar
  2. [2]
    J. D. Wilson, J. Chromatogr.354, 236 (1986).CrossRefGoogle Scholar
  3. [3]
    J. Troke, I. D. Wilson, R. J. Ruane, J. Planar Chromatogr.368, 168 (1986).CrossRefGoogle Scholar
  4. [4]
    G. P. Tomkinson, I. D. Wilson, R. J. Ruane, J. Planar Chromatogr.2, 224 (1989).Google Scholar
  5. [5]
    K. Kovács-Hadady, J. Barna-Katona, J. Planar Chromatogr.2, 133 (1989).Google Scholar
  6. [6]
    K. Kovác-Hadady, J. Szilágyi, J. Chromatogr.553, 459 (1991).CrossRefGoogle Scholar
  7. [7]
    K. Kovács-Hadady, J. Szilágyi J. Planar Chromatogr.4, 194 (1991).Google Scholar
  8. [8]
    K. Kovács-Hadady, J. Chromatogr.589, 301 (1992).CrossRefGoogle Scholar
  9. [9]
    K. Kovács-Hadady, J. Planar Chromatogr.4, 456 (1991).Google Scholar
  10. [10]
    K. Kovács-Hadady, J. Pharm. Biomed. Anal.10, 1025 (1992).CrossRefGoogle Scholar
  11. [11]
    K. Kovács-Hadady, J. Planar Chromatogr.8, 47 (1995).Google Scholar
  12. [12]
    M. Lukács, G. Barcsa, K. Kovács-Hadady, J. Planar Chromatogr.10, 342 (1997).Google Scholar
  13. [13]
    A. E. Martell, R. M. Smith: Critical Stability Constants. Plenum Press. New York-London, 1977.Google Scholar
  14. [14]
    K. Kovács-Hadady, T. Varga, J. Planar Chromatogr.8, 292 (1995).Google Scholar
  15. [15]
    K. Kovács-Hadady, J. Planar Chromatogr.9, 174 (1996).Google Scholar
  16. [16]
    K. Kovács-Hadady, J. Planar Chromatogr.10, 18 (1997).Google Scholar
  17. [17]
    K. Kovács-Hadady, Chromatographia45, 81 (1997).Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1998

Authors and Affiliations

  • M. Lukács
    • 1
  • G. Barcsa
    • 1
  • K. Kovács-Hadady
    • 1
  1. 1.Department of Inorganic and Analytical ChemistryL. Kossuth UniversityDebrecenHungary

Personalised recommendations