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Chromatographia

, Volume 47, Issue 3–4, pp 219–224 | Cite as

Capillary zone electrophoretic separation of basic proteins and drugs using guaran as a buffer modifier

  • Q. Liu
  • F. Lin
  • R. A. Hartwick
Short Communication

Summary

Guaran, a neutral polysaccharide, has been used as a buffer modifier to improve the separation of basic proteins and drugs. Migration reproductibility, peak shape and efficiency were improved when 0.1% guaran was added to the buffer. The concentration of guaran, ionic strength, and pH of buffer solution were optimized to obtain the optimum separation of proteins. Possible separation efficiencies of 700,000 plates per meter were obtained for test proteins. The relative standard deviation (% RSD) of the migration time of all test proteins was less than 0.5%. Improved separation of β-blockers was also observed when guaran was added to the buffer.

Key Words

Capillary electrophoresis Beta-blockers Protein separation Drug separation Guaran Buffer modifier 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1998

Authors and Affiliations

  • Q. Liu
    • 1
  • F. Lin
    • 1
  • R. A. Hartwick
    • 2
  1. 1.P. M. Gross Chemical LaboratoryDuke UniversityDurhamUSA
  2. 2.PharmAssist Analytical Laboratory, Inc.South New BerlinUSA

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