, Volume 47, Issue 5–6, pp 264–270 | Cite as

The application of high performance liquid chromatography, coupled to nuclear magnetic resonance spectroscopy and mass spectrometry (HPLC-NMR-MS), to the characterisation of ibuprofen metabolites from human urine

  • E. Clayton
  • S. Taylor
  • B. Wright
  • I. D. Wilson


The use of HPLC-NMR-MS for the detection and identification of the metabolites of ibuprofen present in a solid phase extract of human urine is described. Gradient reversed-phase HPLC was used to separate the components present in the extract, which were then characterised by a combination of stopped-flow1H NMR and on line electrospray-MS. This approach led to the rapid identification of the known phase 1 human metabolites of ibuprofen, including hydroxy- and carboxy- metabolites, together with their respective glucuronide conjugates. In addition a probable artefact resulting from the dehydration of one of the side chainhydroxylated glucuronides was also identified.

Key Words

Column liquid chromatography NMR and MS detection Ibuprofen metabolites Urine extracts 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1998

Authors and Affiliations

  • E. Clayton
    • 1
  • S. Taylor
    • 1
  • B. Wright
    • 1
  • I. D. Wilson
    • 1
  1. 1.Zeneca Pharmaceuticals, MeresideMacclesfieldUK

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