Summary
Cocaethylene together with cocaine spiked in human whole blood has been found measurable at high sensitivities by capillary gas chromatography with surface ionization detection. The drugs could be rapidly extracted by Sep-Pak C18 cartridges with recovery of more than 60%. The calibration curves for both cocaethylene and cocaine using cocapropylene as internal standard were linear in the range 50–300 pmol mL−1 of whole blood. The detection limits of cocaethylene and cocaine were 5–10 pmol mL−1 (0.1–0.2 pmol on column if recovery is 100%). Cocaethylene could be determined for whole blood obtained from rats (ca. 200 g body wt.), which had received subcutaneous injection of 10 mg cocaine hydrochloride and 2.0 mL of 30% (v/v) ethanol 3 h before sampling; the mean levels of cocaethylene and cocaine were 101 and 1230 pmol mL−1, respectively.
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References
F. K. Rafla, R. L. Epstein, J. Anal. Toxicol.3, 59 (1979).
R. A. Dean, C. D. Christian, R. H. B. Sample, W. F. Bosron, FASEB J,5, 2735 (1991).
D. N. Bailey, J. Anal. Toxicol.18, 13 (1994).
W. L. Hearn, S. Rose, J. Wagner, A. Ciarleglios, D. C. Mash, Pharmacol. Biochem. Behav3, 531 (1991).
F. Moriya, D. T. Anderson, L. K. Chwa, J. J. Muto, T. T. Noguchi, H. Ishizu, Jpn. J. Forensic Toxicol12, 192 (1994).
C.-E. Johanson, C. R. Schuster, in “Psychopharmacology, The Fourth Generation of Progress,” F. E. Bloom, and D. J. Kupfer, eds., Raven Press, New York, 1995, p. 1685.
T. Fujii, H. Arimoto, Anal. Chem57, 2625 (1985).
H. Hattori, T. Yamada, O. Suzuki, J. Chromatogr. A674, 15. (1994).
S. P. Findlay, J. Am. Chem. Soc.76, 2855 (1954).
I. M. Roy, T. M. Jefferies, M. D. Threadgill, G. H. Dewar, J. Pharm. Biomed. Anal.10, 943 (1992).
D. N. Bailey, J. Anal. Toxicol.17, 79 (1993).
G. W. Hime, W. L. Hearn, S. Rose, J. Cofino, J. Anal. Toxicol.15, 241 (1991).
A. H. B. Wu, T. A. Onigbinde, K. G. Johnson, G. H. Wimbish, J. Anal. Toxicol.16, 132 (1992).
G. M. Abusada, I. K. Abukhalaf, D. D. Alford, I. Vinzon-Bautista A. K. Pramanik, N. A. Ansari, J. E. Manno, B. R. Manno, J. Anal. Toxicol.17, 353 (1993).
W.-L. Wang, W. D. Darwin, E. J. Cone, J. Chromatogr. B660, 279 (1994).
S. M. Roberts, J. W. Munson, R. C. James, R. D. Harbison, Anal. Biochem.202, 256 (1992).
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Watanabe, K., Hattori, H., Nishikawa, M. et al. Simultaneous determination of cocaethylene and cocaine in blood by gas chromatography with surface ionization detection. Chromatographia 44, 55–58 (1997). https://doi.org/10.1007/BF02466516
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DOI: https://doi.org/10.1007/BF02466516