Summary
The analysis of sterols in vegetable oils by off-line SFC followed by capillary GC-MS is described. The fractionation of the sterols from the complex oil matrix is achieved by SFC on aminopropyl silicagel in less than 8 minutes. Injection and collection of the sterol fraction is fully automated and time controlled. The sterols are analysed without derivatisation by capillary GC-MS. Identification is performed by full scan electron ionisation and quantitation is carried out by extracted ion chromatography at m/e 107, with cholesterol as internal standard. The analyses of the sterols from the sunflower oil and two olive oils illustrate the possibilities of the method.
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References
N. Frega, F. Bocci, G. Lercker, J. Am. Oil Chem. Soc.69, 447 (1992).
M. Noda, M. Tanaka, Y. Seto, T. Aiba, C. Oku, Lipids23, 439 (1988).
N. Frega, F. Bocci, G. Lercker, J. Am. Oil Chem. Soc.70 919 (1993).
K. Grob, M. Lanfranchi, C. Mariani, J. Chromatogr.471, 397 (1989).
K. Grob andM. Lanfranchi HRC12, 624 (1989).
C. Mariani, K. Grob, M. Lanfranchi, J. Am. Oil Chem. Soc.,67, 626 (1990).
F. David, A. Medvedovici, P. Sandra, in preparation.
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Medvedovici, A., David, F. & Sandra, P. Analysis of sterols in vegetable oils using off-line SFC/capillary GC-MS. Chromatographia 44, 37–42 (1997). https://doi.org/10.1007/BF02466513
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DOI: https://doi.org/10.1007/BF02466513