Summary
For the analysis of pesticides such as isoproturon in complex matrices the rate determining step is sample preparation. A novel solid-phase extraction system for isoproturon based on antibody mediated extraction has been developed. The isoproturon can be retained while the immuno-extraction column is washed with phosphate buffered saline and eluted using phosphate buffered saline/ethanol at low pH. The column can preconcentrate isoproturon from up to at least 1000 mL water and elute in 1 mL. Quantitative recovery of isoproturon from water, plasma and urine can be obtained and the proposed water assay can detect as low as 50 ng L−1 with a 50 mL sample, or 5 ng L−1 with a 1 L sample.
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Shahtaheri, S.J., Kwasowski, P. & Stevenson, D. Highly selective antibody-mediated extraction of isoproturon from complex matrices. Chromatographia 47, 453–456 (1998). https://doi.org/10.1007/BF02466480
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DOI: https://doi.org/10.1007/BF02466480