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Aminopropyl-silica as an advantageous alternative to nonpolar sorbents for continuous cleanup/preconcentration of vitamin D3 metabolites

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Summary

A new procedure for continuous cleanup and concentration of hydroxyvitamin D3 metabolites prior to their separation by HPLC and UV-detection is reported. The process is based on the use of aminopropyl-silica as solid-phase sorbent as an alternative to the use of nonpolar sorbents. The improvement thus achieved has been tested by comparing the results with those obtained using octadecyl-C18 as non-polar sorbent. The comparison has been based on the calibration graphs (linear range, detection and quantitation limits), precision and multiple standard addition method.

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Abbreviations

25-(OH)-D3 :

25-hydroxyvitamin D3

24,25-(OH)2-D3 :

24,25-dihydroxyvitamin D3

1,25-(OH)2-D3 :

1,25-didydroxyvitamin D3

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, 14004, Córdoba, Spain

    F. Ortiz Boyer, J. M. Fernández Romero & M. D. Luque de Castro

  2. Mineral Metabolism Unit, Hospital Reina Sofía, University of Córdoba, 14004, Córdoba, Spain

    J. M. Quesada

Authors
  1. F. Ortiz Boyer
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  2. J. M. Fernández Romero
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  3. M. D. Luque de Castro
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  4. J. M. Quesada
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Boyer, F.O., Fernández Romero, J.M., Luque de Castro, M.D. et al. Aminopropyl-silica as an advantageous alternative to nonpolar sorbents for continuous cleanup/preconcentration of vitamin D3 metabolites. Chromatographia 47, 367–372 (1998). https://doi.org/10.1007/BF02466466

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  • DOI: https://doi.org/10.1007/BF02466466

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