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Chromatographia

, Volume 44, Issue 3–4, pp 172–178 | Cite as

A quantitative approach to the analysis of supermicron dispersions by field-flow fractionation with UV-vis detectors. The application of an absolute method

  • P. Reschiglian
  • D. Melucci
  • G. Torsi
Originals

Summary

Quantitative analysis in field-flow fractionation is becoming a necessary requirement for routine applications, instrumental optimization and scale-up to preparative separations. The use of detection systems which show complex dependence on sample characteristics (i.e. UV spectrometry) has hindered the application of quantitative methods of analysis in field-flow fractionation. A standardless model, shown valid in flow-through, homogeneous systems, is applied here to a heterogeneous system (dispersed supermicron particles) in field-flow fractionation by single peak area measurements. Absolute analysis in the fractionation of spherical silica particles for high-performance liquid chromatography column packing by gravitational field-flow fractionation with UV-Vis detectors is presented. It has been shown that for such samples extinction coefficients are independent of sample concentration and are determined by the size and density of the particles. The accuracy of such an approach to absolute analysis is discussed.

Key Words

Field-flow fractionation Particulate matter analysis Turbidimetric detection Analysis without standards 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1997

Authors and Affiliations

  • P. Reschiglian
    • 1
  • D. Melucci
    • 1
  • G. Torsi
    • 1
  1. 1.Department of Chemistry “G. Ciamician”University of BolognaBolognaItaly

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