Skip to main content
Log in

Differential scanning calorimetric analysis of fruit-body and mycelium ofLentinula edodes

  • Short Communication
  • Published:
Mycoscience

Abstract

Differential scanning calorimetric (DSC) analysis was applied to the fruit-body (pileus and stipe) and mycelium, of shiitake mushroom (Lentinula edodes). Thermograms of each sample indicated distinctive patterns. However, chemical and infrared (IR) spectroscopic analyses showed that the compositions of pileus and stipe were similar to each other and different from that of mycelium. Because the DSC thermogram depends not only on chemical composition but also on physical properties such as density, the result of DSC analysis was assumed to indicate a difference in the state of cell wall between pileus and stipe.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Literature cited

  • Aoyagi, Y., Kasuga, A., Sasaki, H., Matuzawa, M., Tsutagawa, Y. and Kawai, H. 1993. Chemical compositions of Shiitake mushroom [Lentinus edodes (Berk.) Sing.] cultivated on logs and sawdust substrate beds and their relations to composition of the substrate. Nippon Shokukin Kogyo Gakkaishi40: 771–775. (In Japanese.)

    CAS  Google Scholar 

  • Bartnicki-Gracia, S. and Nickerson, W. J. 1962. Isolation, composition, and structure of cell walls of filamentous and yeas-like forms ofMucor rouxii. Biochim. Biophys. Acta58: 102–119.

    Article  Google Scholar 

  • Bitter, T. and Muir, M. H. 1962. A modified uronic acid and carbazole reaction. Anal. Biochem.4: 300–334.

    Article  Google Scholar 

  • Iiyama, K. and Wallis, A. F. A. 1988. An improved acetyl bromide procedure for determinating lignin in woods and wood pulps. Wood Sci. Technol.22: 271–280.

    Article  CAS  Google Scholar 

  • Kawamura, N., Goto, M. and Nakamura, Y. 1983. Effect of lignin and its precursors on vegetative growth and fruiting body formation inLentinus edodes. Trans. Mycol. Soc. Japan24: 213–222. (In Japanese.)

    CAS  Google Scholar 

  • Kurasawa, S., Sugahara, T. and Hayashi, J. 1982. Proximate and dietary fibre analysis of mushrooms. Nippon Shokuhin Kogyo Gakkaishi29: 400–406. (In Japanese.)

    CAS  Google Scholar 

  • Mitchell, B. D. 1957. Differential thermal characteristics of peat. Nature180: 1414–1415.

    Article  CAS  Google Scholar 

  • Mitchell, B. D. 1969. The differential thermal analysis of humic substances and related materials. Sci. Proc. R. Dublin Soc.A1: 105–114.

    Google Scholar 

  • Mitchell, B. D. and Knight A. H. 1965. The application of differential thermal analysis to plant materials. J. Exp. Bot.16: 1–15.

    Google Scholar 

  • Prieto, A., Bernabé, M. and Leal, J. A. 1995. Isolation, purification and chemical characterization of alkali-extractable polysaccharides from the cell walls ofTalaromyces species. Mycol. Res.99: 69–75.

    Article  CAS  Google Scholar 

  • Reh, U., Kraepelin, G. and Lamprecht, I. 1986. Use of differential scanning calorimetry for structural analysis of fungally degraded wood. Appl. Environ. Microbiol.52: 1101–1106.

    PubMed  CAS  Google Scholar 

  • Saeman, J. F., Moore, W. E., Mitchell, R. L. and Millett, M. A. 1954. Techniques for the determination of pulp constituents by quantitative paper chromatography. TAPPI37: 336–343.

    CAS  Google Scholar 

  • Sangar, V. K. and Dugan, P. 1973. Chemical composition of the cell wall ofSmittium culisetae (Trichomycetes). Mycologia65: 421–431.

    CAS  Google Scholar 

  • Sawada, M. 1965. Studies on chemical components of wild mushrooms and toadstools in Japan. Bull. Tokyo Univ. Forests59: 33–169. (In Japanese.)

    Google Scholar 

  • Stewart, J. M., Birnie, A. C. and Mitchell, B. D. 1966. The characterization of a peat profile by thermal methods. Agrochimica11: 92–104.

    Google Scholar 

  • Tsujiyama, S. 1996. Degradation of lignin-carbohydrate complex by wood-rotting fungi. VI. Detection of changes of lignin-carbohydrate matrix by differential scanning calorimeter (DSC). Proc. 41st Lignin Sym., Nagoya Japan, pp. 117–120. (In Japanese.)

  • Yoshida, H., Sugahara, T. and Hayashi, J. 1982. Studies on free sugars, free sugaralcohols and organic acids of edible mushrooms. Nippon Shokuhin Kogyo Gakkaishi29: 451–459. (In Japanese.)

    CAS  Google Scholar 

  • Yoshida, H., Sugahara, T. and Hayashi, J. 1986a. Changes in the contents of carbohydrates and organic acids in fruitbodies of Shiitake-mushroom [Lentinus edodes (Berk.) Sing.] during development and post-harvest storage. Nippon Shokuhin Kygyo Gakkaishi33: 414–425. (In Japanese.)

    CAS  Google Scholar 

  • Yoshida, H., Sugahara, T. and Hayashi, J. 1986b. Studies on free sugars, free sugaralcohols and organic acids of wild mushrooms. Nippon Shokuhin Kogyo Gakkaishi33: 426–433. (In Japanese.)

    CAS  Google Scholar 

  • Yoshida, H., Sugahara, T. and Hayashi, J. 1987. Changes in carbohydrates and organic acids during development of mycelia and fruit-bodies of Shiitake mushroom [Lentinus edodes (Berk.) Sing.]. Nippon Shokuhin Kogyo Gakkaishi34: 274–281. (In Japanese.)

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

About this article

Cite this article

Tsujiyama, Si. Differential scanning calorimetric analysis of fruit-body and mycelium ofLentinula edodes . Mycoscience 40, 69–72 (1999). https://doi.org/10.1007/BF02465676

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02465676

Key Words

Navigation