, Volume 40, Issue 1, pp 35–39 | Cite as

Grouping of isolates in AG 2 ofRhizoctonia solani by total cellular fatty acid analysis

  • Masaru Matsumoto
  • Nobuaki Matsuyama
Original Papers


Total-cellular fatty acid compositions of 34 isolates ofRhizoctonia solani belonging to intraspecific groups (ISGs) of anastomosis group (AG) 2, i.e., AG 2-1, AG 2-2 IIIB (mat rush), AG 2-2 IV (sugar beet), AG 2-2 LP (turfgrass), and AG 2–3 (soybean), were compared. The major fatty acids identified were palmitic, stearic, and oleic acids. Principal component analysis based on the percentage composition of total cellular fatty acids revealed consistently low variability among isolates of a single ISG of AG 2. Average linkage cluster analysis showed that isolates obtained from turfgrass representing a newly proposed group, AG 2-2 LP, were differentiated from other AG 2 ISGs. Isolates of another newly proposed group AG 2–3, from diseased soybean were also closely related to AG 2-1 and AG 2-2 IIIB but distinguishable from the AG 2-1 and AG 2-2 LP isolates by the average linkage cluster analysis. These results suggested that the percentage composition of total-cellular fatty acids is a distinct characteristic for the five ISGs belonging to AG 2, and fatty acid analysis is useful for the differentiation and characterization of these ISGs of AG 2 inR. solani.

Key Words

fatty acid analysis PCR-RFLP analysis Rhizoctonia solani AG 2 


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Copyright information

© The Mycological Society of Japan 1999

Authors and Affiliations

  • Masaru Matsumoto
    • 1
  • Nobuaki Matsuyama
    • 2
  1. 1.Institute of Tropical AgricultureKyushu UniversityFukuokaJapan
  2. 2.Laboratory of Plant Pathology, Faculty of AgricultureKyushu UniversityFukuokaJapan

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