Abstract
The effects of vasopressin on hippocampal slices from control and pretrained rats were compared. Prolonged presentation of both combined and noncombined conditioned stimuli (CS) and unconditioned stimuli (US) increases the sensitivity of hippocampal neurons to vasopressin. The effect of this was that responses to application of DG-AVP after training and active control treatment was biphasic, consisting of initial excitation and subsequent inhibition; similar effects were seen in control slices when the peptide concentration was increased. Increases in the excitatory phase occurred with presentation of the CS and US and were independent of the training factor. The inhibitory phase increased to a greater extent on presentation of combinations of the CS and US. It is suggested that increases in depression in response to application of vasopressin in trained animals, as compared with active controls, are due to a higher level of free intracellular calcium occurring as a result of the training procedure.
Similar content being viewed by others
References
R. I. Kruglikov, “Main trends in studies of the neurochemical mechanisms of learning and memory,”Zh. Vyssh. Nerv. Deyat.,36, No. 2, 226 (1986).
I. E. Kudryashov, “The potentiating effect of vasopressin is not blocked by ketamine,”Zh. Vyssh. Nerv. Deyat.,44, No. 4-5, 862 (1994).
I. E. Kudryashov and I. M. Ivanova, “Studies of the mechanisms of the effect of a vasopressin analog on the efficiency of glutamatergic transmission in the hippocampus,”Zh. Vyssh. Nerv. Deyat.,45, No. 3, 589 (1995).
O. S. Papsuevich, G. I. Chipens, and S. V. Mikhailev,Neurohypophyseal Hormones [in Russian], Zinatne, Riga (1986).
A. I. Chepkov, “The effect of vasopressin on the properties of long-term postsynaptic potentiation in hippocampal slices,”Zh. Vyssh. Nerv. Deyat.,31, No. 2, 45 (1981).
R. D. Brinton, T. M. Gonzalez, and W. S. Cheung, “Vasopressin-induced calcium signaling in cultured hippocampal neurons,”Brain Res.,667, 151 (1994).
R. M. Buijs, “Vasopressin and oxytocin—their role in neurotransmission,”Pharmacol. Therp.,22, 127 (1983).
C. Chen, R. D. Diaz-Brinton, T. J. Shors, and R. F. Thompson, “Vasopressin induction of long-lasting potentiation of synaptic transmission in the dentate gyrus,”Hippocampus,3, 193 (1993).
A. F. Crine and R. M. Buijs, “Electric footshocks differentially affect plasma and spinal cord vasopressin and oxytocin levels,”Peptides,8, 243 (1987).
E. R. de Kloet, F. Rotteveel, T. A. M. Voorhuis, and M. Terlou, “Topography of binding sites for neurohypophyseal in rat brain,”Eur. J. Pharmacol.,110, 113 (1985).
D. De Wied, O. Gaffori, J. M. van Ree, and W. De Jong, “Central targets for the behavioural effects of vasopressin neuropeptides,”Nature,308, 276 (1984).
J. Dogterom and R. M. Buijs, “Vasopressin and oxytocin. Distribution in rat brain: radioimmunoassay and immunocytochemical studies,” in:Peptides and Neural Transmission, D. M. Gash and G. J. Boer (eds.), Raven Press (1987), p. 307.
A. Ermish, R. Landgraf, and P. Mobius, “Vasopressin and oxytocin in brain areas of rats with high or low behavioural performance,”Brain Res.,379, 24 (1986).
A. Hirasawa, Y. Nakayama, N. Ishiharada, et al., “Evidence for the existence of vasopressin V2 receptor mRNA in rat hippocampus,”Biochem. Biophys. Res. Commun.,205, 1702 (1994).
L. L. Iverwen, “Function and distribution of peptides in the nervous system,”Biochem. Soc. Trans.,13, 35 (1985).
M. Joels and I. J. A. Urban, “The effect of microiontophoretically applied vasopressin and oxytocin on single neurones in the septum and dorsal hippocampus of the rat,”Neurosci. Lett.,33, 79 (1982).
I. V. Kudryashova, “Frequency facilitation in the slices of conditioned rats,”Neurosci.,75, 695 (1996).
F. Laczi, O. Gaffory, E. R. De Kloet, and D. Wied, “Differential regulation in immunoreactive arginine vasopressin content of microdissected brain region during passive avoidance behaviour,”Brain Res.,260, 342 (1983).
C. Lebrun, M. Le Moal, R. Dantzer, et al., “Hypertonic saline mimics the effects of vasopressin on inhibitory avoidance in the rat,”Behav. Neural. Biol.,47, 130 (1987).
G. Lynch and U. Staubli, “Possible contributions of longterm potentiation to the encoding and organization of memory,”Brain Res. Rev.,16, 193 (1991).
I. M. Petracca, B. G. Baskin, J. Diar, and D. M. Dorsa, “Ontogenic changes in vasopressin binding site distribution in rat brain: an autoradiographic study,”Develop. Brain Res.,28, 63 (1986).
J. Raber, E. M. Pich, G. F. Koob, and F. E. Bloom, “IL-1 beta potentiates the acetylcholine-induced release of vasopressin from the hypothalamus in vitro, but not from the amygdala,”Neuroendocrinology,59, 208 (1994).
X. W. Rong, X. F. Chen, and Y. C. Du, “Potentiation of synaptic transmission by neuropeptide AVP4–8 (ZNC(C)PR) in rat hippocampal slices,”Neuroreport.,4, 1135 (1993).
B. E. Tiberiis, H. McLennan, and N. Wilson, “Neurohypophyseal peptides and the hippocampus. II. Excitation of rat hippocampal neurones by oxytocin and vasopressin applied in vitro,”Neuropeptides,4, 73 (1983).
F. W. van Leeuwen, “Vasopressin receptors in the brain and pituitary,” in:Neuropeptides and Neural Transmission D. M. Gash and G. J. Boer (eds.), Raven Press, New York (1987), p. 477.
Author information
Authors and Affiliations
Additional information
Translated from Zhurnal Vysshei Nervnoi Deyatel'nosti, Vol. 48, No. 2, pp. 222–228, March–April, 1998.
Rights and permissions
About this article
Cite this article
Kudryashova, I.V., Kudryashov, I.E. The effects of vasopressin on hippocampal slices from trained rats. Neurosci Behav Physiol 29, 289–293 (1999). https://doi.org/10.1007/BF02465340
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02465340