Summary
Eight representative recombinant background clones of λEMBL3 were analysed usingKpnI,BamHI,SalI,EcoRI andHindIII digestion. We found that λEMBL3 carries its own left arm in theBamHI cloning site. In this way, recombinant molecules were found to be generated which can grow onEscherichia coli strain NM539. In all cases analysed, the left arm DNA was inserted in a head to tail orientation. Seven clones carried a restoredBamHI site at thecos site-BamHI site connection. In the region where the inserted left arm and the right arm were ligated,BamHI cloning produces a large palindromic sequence consisting of two polylinkers. ThisBamHI site was incompletely cleaved in all cases analysed. We assume that a part of the λ DNA molecule in this region shows a cruciform structure prohibiting recognition or cleavage of this site by restriction endonucleaseBamHI.
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Communicated by E. Bautz
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Tröster, H., Kissel, W., Trendelenburg, M.F. et al. Restriction analysis of λEMBL3 background recombinants: Occurrence of λ phages carrying a head to tail oriented left arm DNA sequence. Molec. Gen. Genet. 217, 533–535 (1989). https://doi.org/10.1007/BF02464928
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DOI: https://doi.org/10.1007/BF02464928