Summary
Strains carrying plasmids that code for 10Sa RNA synthesize a larger molecule when the RNA processing enzyme RNase E is inactivated. The T1 fingerprint of 10Sa RNA and the larger molecule is very similar, but the latter contains additional oligonucleotides. We show that the larger RNA is converted to the smaller, mature RNA. The precursor molecule starts with an adenosine triphosphate and is therefore a primary transcript. RNase E is not the enzyme that processes p10Sa (precursor 10Sa) RNA into 10Sa RNA. The cell extract contains an activity that carries out this conversion. This activity requires the dication Mn2+.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bolivar F, Rodriquez RL, Green PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Folkow S (1977) Construction and characterization of new cloning vehicles. II. Multipurpose cloning system. Gene 2: 95–113
Chen SC, Abelson J (1986) Fractionation and characterization of a yeast mRNA splicing extract. Proc Natl Acad Sci USA 83: 2387–2391
Gegenheimer P, Apirion D (1978) Processing of ribosomal RNA by RNase P: Spacer tRNAs are linked to 16S rRNA in an RNase P RNase III mutant strain ofE. coli. Cell 15: 527–539
Gegenheimer P, Apirion D (1980) Structural characterization andin vitro processing ofEscherichia coli ribosomal RNA transcripts containing 5′ triphosphates, leader sequences, 16S RNA and spacer tRNAs. J Mol Biol 143: 227–257
Gegenheimer P, Watson N, Apirion D (1977) Multiple pathways for primary processing of ribosomal RNA inEscherichia coli. J Biol Chem 252: 3064–3073
Ghora BK, Apirion D (1978) Structural analysis andin vitro processing to p5 rRNA of a 9S RNA molecule isolated from anrne mutant ofEscherichia coli. Cell 15: 1055–1066
Goldblum K, Apirion D (1981) Inactivation of the RNA processing enzyme ribonuclease E blocks cell division. J Bacteriol 146: 128–132
Goldstein J, Harwood K (1969) Another species of ribonucleic acid inEscherichia coli. J Mol Biol 39: 383–387
Griffin BE (1971) Separation of32P-labeled ribonucleic acic components. The use of polyethylenimine-cellulose (TLC) as a second dimension in separating oligoribonucleotides of ‘4.5S’ and 5S fromE. coli. FEBS Lett 15: 165–168
Griffin BE (1975) Studies and sequences ofEscherichia coli 4.5S RNA. J Biol Chem 250: 5426–5437
Grunstein M, Hogness D (1975) Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72: 3961–3965
Guerrier-Takada C, Gardiner K, Marsh T, Pace N, Altman S (1983) The RNA moiety of Ribonuclease P is the catalytic subunit of the enzyme. Cell 35: 849–857
Gurevitz M, Jain SK, Apirion D (1983) Identification of a precursor molecule for the RNA moiety of the processing enzyme RNase P. Proc Natl Acad Sci USA 80: 4450–4454
Hindley J (1967) Fractionation of32P-labeled ribonucleic acids on polyacrylamide gels and their characterization by finger printing. J Mol Biol 30: 125–136
Ikemura T, Nomura M (1977) Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hyvrid ColE1 plasmids inE. coli. Cell 11: 779–793
Jain SK, Gurevitz M, Apirion M (1982a) A small RNA that complements mutants in the RNA processing enzyme ribonuclease P. J Mol Biol 162: 515–533
Jain SK, Pragai B, Apirion D (1982b) A possible complex containing RNA processing enzymes. Biochem Biophys Res Commun 106: 768–778
Lee SY, Bailey SC, Apirion D (1978) Small stable RNAs fromEscherichia coli: evidence for the existence of new molecules and for a new ribonucleoprotein particle containing 6S RNA. J Bacteriol 133: 1015–1023
Maniatis T, Reed R (1987) The roll of small nuclear ribonucleoproteins particles in pre-mRNA splicing. Nature 325: 673–678
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Misra TK, Apirion D (1980) Generne affects the structure of the ribonucleic acid processing enzyme ribonuclease E ofEscherichia coli. J Bacteriol 142: 359–361
Pragai B, Apirion D (1982) Processing of bacteriophage T4 transfer RNAs, structural analysis andin vitro processing of precursors that accumulate in RNase E− strains. J Mol Biol 154: 465–484
Ray BK, Apirion D (1979) Characterization of 10Sa RNA: a new stable RNA molecule fromEscherichia coli. Mol Gen Genet 174: 25–32
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–509
Szeberenyi J, Apirion D (1983) Initiation processing and termination of ribosomal RNA from a hybrid 5S ribosomal RNA gene in a plasmid. J Mol Biol 168: 525–561
Thomas PS (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77: 5201–5205
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Subbarao, M.N., Apirion, D. A precursor for a small stable RNA (10Sa RNA) ofEscherichia coli . Molec. Gen. Genet. 217, 499–504 (1989). https://doi.org/10.1007/BF02464923
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02464923