Molecular and General Genetics MGG

, Volume 217, Issue 2–3, pp 464–470 | Cite as

Chromatin structure of the 5′ flanking region of the yeastLEU2 gene

  • J. F. Martínez-García
  • F. Estruch
  • J. E. Pérez-Ortín
Article

Summary

The chromatin structure of theLEU2 gene and its flanks has been studied by means of nuclease digestion, both with micrococcal nuclease and DNase I. The gene is organized in an array of positioned nucleosomes. Within the promoter region, the nucleosome positioning places the regulatory sequences, putative TATA box and upstream activator sequence outside the nucleosomal cores. The tRNA3 Leu gene possesses a characteristic structure and is protected against nucleases. Most of the 5′ flank is sensitive to DNase I digestion, although no clear hypersensitive sites were found. The chromatin structure is independent of either the transcriptional state of the gene or the chromosomal or episomal location. Finally, in the plasmid pJDB207, which lacks most of the promoter, we have found that the chromatin structure of the coding region is similar to that of the wild-type allele.

Key words

Nucleosome positioning LEU2 gene Saccharomyces cerevisiae tRNA3Leu gene Chromatin 

Abbreviations

MNase

micrococcal nuclease

ORF

open reading frame

UAS

upstream activator sequence

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • J. F. Martínez-García
    • 1
  • F. Estruch
    • 1
  • J. E. Pérez-Ortín
    • 1
  1. 1.Departmento de Bioquímica y Biología Molecular, Facultades de CienciasUniversitat de ValènciaValènciaSpain

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