Summary
The receptive component of theCy transposable element system (rcy: Mu7) at theBz locus ofZea mays L. is 2.2 kb and has long terminal inverted repeats. The insertion is flanked by a 9 bp duplication. In the presence of an autonomousCy element in the genome,rcy: Mu7 is excised frombz-rcy in a manner consistent with a model suggested previously. The termini ofrcy: Mu7 have 85% sequence similarity with theMu1 element ofZ. mays. This is consistent with the observation thatMu1 can behave genetically like a receptive component of theCy system.
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References
Barker RF, Thompson DV, Talbot DR, Swanson J, Bennetzen JL (1984) Nucleotide sequence of the maize transposable elementMu1. Nucleic Acids Res 12:5955–5967
Benton W, Davis R (1977) Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 196:180–182
Davis RW, Hyman RW (1971) A study in evolution: the DNA base sequence homology between Coliphages T7 and T3. J Mol Biol 62:287–301
Davis RW, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods Enzymol 21:413–428
Dooner HK, (1981) Regulation of the enzyme UFGT by the controlling elementDs inbz-m4, an unstable mutant in maize. Cold Spring Harbor Symp Quant Biol 45:457–462
Dooner HK, Nelson OE Jr (1979) Heterogenous flavonoid glucosyltransferases in purple derivatives from a controlling element-suppressedbronze mutant in maize. Proc Natl Acad Sci USA 76:2369–2371
Fedoroff NV, Furtek DB, Nelson OE Jr (1984) Cloning of thebronze locus in maize by a simple and generalizable procedure using the transposable controlling elementActivator (Ac). Proc Natl Acad Sci USA 81:3825–3829
Frischauf A-M, Lehrach H, Poustka A, Murray N (1983) Lambda replacement vectors carrying polylinker sequences. J Mol Biol 170:827–842
Furtek D, Schiefelbein JW, Johnston F, Nelson OE Jr (1988) Sequence comparisons of three wild-typeBronze-1 alleles fromZea mays. Plant Mol Biol 11:473–481
Kim H-Y, Schiefelbein JW, Raboy V, Furtek DB, Nelson OE Jr (1987) RNA splicing permits expression of a maize gene with a defective Suppressor-mutator transposable element insertion in an exon. Proc Natl Acad Sci USA 84:5863–5867
Lillis M, Freeling M (1986)Mu transposons in maize. Trends Genet 2:183–188
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Maxam AM, Gilbert W (1980) Sequencing end-labelled DNA with base specific chemical cleavages. Methods Enzymol 65:499–580
Murray NE (1983) Phage lambda and molecular cloning. In: RW Hendrix (ed) The Bacteriophage Lambda II, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 395–432
Nevers P, Shepherd NS, Saedler H (1985) Plant transposable elements. Adv Bot Res 12:102–203
O’Reilly C, Shepherd NS, Pereira A, Schwarz-Sommer Zs, Bertram I, Peterson PA, Saedler H (1985) Molecular cloning of thea1 locus ofZea mays using the transposable elementsEn andMu1. EMBO J 4:877–882
Peterson PA (1988a)a2-m668291 — linkage ofCy withpr. Maize Genet Coop Newslett 62:3
Ralston EJ, English JJ, Dooner HK (1988) Sequence of threebronze alleles of maize and correlations with the genetic fine structure. Genetics 119:185–197
Robertson DS (1978) Characterization of a mutator system in maize. Mutat Res 51:21–28
Robertson DS (1983) A possible dose-dependent inactivation ofMutator (Mu) in maize. Mol Gen Genet 191:86–90
Robertson DS (1986) Genetic studies on the loss ofMu mutator activity. Genetics 113:765–773
Saedler H, Nevers P (1985) Transposition in plants: a molecular model. EMBO J 4:585–590
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Schnable PS (1986) Genetic characterization of theCy transposable element system at theBz locus ofZea mays L. Unpublished Ph.D. dissertation. Iowa State University, Ames, Iowa, USA
Schnable PS, Peterson PA (1986) Distribution of genetically activeCy elements among diverse maize lines. Maydica 31:59–81
Schnable PS, Peterson PA (1988) TheMutator-relatedCy transposable element ofZea mays L. behaves as a near-Mendelian factor. Genetics 120:587–596
Schnable PS, Peterson PA (1989) Genetic evidence of a relationship between two maize transposable element systems:Cy andMutator. Mol Gen Genet 215:317–321
Schwarz-Sommer ZS, Gierl A, Klösgen RB, Wienand U, Peterson PA, Saedler H (1984) The Spm (En) transposable element controls the excision of a 2-kb DNA insert at thewx-m8 allele ofZea mays. EMBO J 3:1021–2028
Shapiro MB, Senapathy P (1987) RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucleic Acids Res 15:7155–7174
Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Sundaresan V, Freeling M (1987) An extrachromosomal form of theMu transposons of maize. Proc Natl Acad Sci USA 84:4924–4928
Talbert LE, Kelly AJ, Chandler VL (1988) Characterization of a class ofMu elements present in normal lines. Maize Genet Coop Newslett 62:59
Taylor LP, Walbot V (1987) Isolation and characterization of a 1.7-kb transposable element from Mutator line of maize. Genetics 117:297–307
Yamamoto KR, Alberts BM, Benzinger R, Lawhorne K, Treiber G (1970) Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
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Schnable, P.S., Peterson, P.A. & Saedler, H. Thebz-rcy allele of theCy transposable element system ofZea mays contains aMu-like element insertion. Molec. Gen. Genet. 217, 459–463 (1989). https://doi.org/10.1007/BF02464917
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DOI: https://doi.org/10.1007/BF02464917