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Heterogeneous genomic amplification inStreptomyces glaucescens: Structure, location and junction sequence analysis

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Summary

Certain chromosomal markers inStreptomyces glaucescens behave unstably, being lost at high frequency as a result of extensive genomic deletion. Additionally, mutant strains possessing such deletions frequently display intense DNA amplification. With the help of a wild-type cosmid library we investigated the structure of the amplified DNA sequences (ADS) and the corresponding wild-type amplifiable units of DNA (AUD). The reiterations were heterogeneous in location, copy number and sequences involved and originated predominantly from a single 100 kb region of the chromosome called the AUD locus. All strains bearing reiterations possessed associated deletions which terminated either close to or at the ADS. The termini of four AUDs were sequenced in order to gain more knowledge about these heterogeneous amplifications. In three of the four cases investigated small, interrupted homologies were found bordering the AUDs. With the help of orthogonal-field-alternation gel electrophoresis (OFAGE) we were able to visualize a tandem reiteration of more than 1500 kb in length.

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Author notes

  1. Wilhelm Krek

    Present address: ISREC (Institut suisse de recherches expérimentales sur le cancer), 1066, Epalinges, Switzerland

  2. Jacqueline Piret

    Present address: Department of Biology, Northeastern University, Boston, Mass., USA

Authors and Affiliations

  1. Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, CH-8092, Zürich, Switzerland

    Alex Häusler, Ashley Birch, Wilhelm Krek, Jacqueline Piret & Ralf Hütter

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  1. Alex Häusler
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  2. Ashley Birch
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  3. Wilhelm Krek
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  4. Jacqueline Piret
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  5. Ralf Hütter
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Communicated by H. Hennecke

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Häusler, A., Birch, A., Krek, W. et al. Heterogeneous genomic amplification inStreptomyces glaucescens: Structure, location and junction sequence analysis. Molec. Gen. Genet. 217, 437–446 (1989). https://doi.org/10.1007/BF02464915

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  • DOI: https://doi.org/10.1007/BF02464915

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