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Molecular and General Genetics MGG

, Volume 217, Issue 2–3, pp 257–262 | Cite as

A new mutant class of soybean lacks urease in leaves but not in leaf-derived callus or in roots

  • Joseph C. Polacco
  • Adam K. Judd
  • Jody K. Dybing
  • Silvia R. Cianzio
Article

Summary

We reported earlier the recovery of two classes of soybean urease mutants in soybean (Glycine max L. Merr. cv. Williams). Class I mutants lack the embryo-specific urease while class II mutants lack the activities of both urease isozymes, the embryo-specific and the ubiquitous urease, the latter found in all tissues examined. We report here the recovery of a true-breeding mutant, aj3, which represents the third phenotypic class: normal levels of embryo-specific urease and little or no ubiquitous urease. Unlike class II mutant plants which lack urease in all tissue, aj3 lacks urease activity only in leaves (ca. 2% normal activity); its roots have near normal urease activity. Callus derived from leaves of aj3 has 14% to 40% the urease activity of Williams 82 callus. This partial reduction in urease activity in aj3 callus is sufficient to reduce growth with urea as sole nitrogen source and to confer resistance to 50 mM urea added to callus maintenance medium. Leaves of aj3 produce more than 40 times the urease antigen expected from their urease activity. The aj3 trait is due to a single recessive lesion which is not allelic with lesions at theEu2, Eu3 (class II) orEu1 (class I) loci. We designate the aj3 genotype aseu4/eu4.

Key words

Soybean mutant Ubiquitous urease Callus Leaf Root 

Abbreviations

ABTS

2,2′-azino-di[3-ethylbenzthiazolinesulfonic acid] diammonium salt

BSA

bovine serum albumin

ELISA

enzyme-linked immunosorbent assay

EMS

ethyl methanesulfonate

MS

Murashige and Skoog medium

NMU

N-methyl nitrosourea

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Joseph C. Polacco
    • 1
  • Adam K. Judd
    • 1
  • Jody K. Dybing
    • 1
  • Silvia R. Cianzio
    • 2
  1. 1.Biochemistry DepartmentUniversity of MissouriColumbiaUSA
  2. 2.Agronomy DepartmentIowa State UniversityAmesUSA

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