Mycoscience

, Volume 39, Issue 3, pp 273–278 | Cite as

Isolation of a gram-positive bacterium effective in suppression of brown blotch disease of cultivated mushrooms,Pleurotus ostreatus andAgaricus bisporus, caused byPseudomonas tolaasii

  • Takanori Tsukamoto
  • Akira Shirata
  • Hitoshi Murata
Original Papers

Abstract

A Gram-positive bacterium was isolated from a rottingPleurotus ostreatus fruiting body that markedly reduced the level of extracellular toxins (i.e., tolaasins) produced byPseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. The isolated bacterium is saprophytic but not parasitic nor pathogenic toP. ostreatus. A low ratio, ca. 10−3 cells of the isolated bacterium for oneP. tolaasii cells, was sufficient for detoxification in vitro. Inoculation of the isolated bacterium prevents the development of bacterial disease inP. ostreatus andAgaricus bisporus. The suppression of the disease development, however requires the initial cell density equivalent to ca. 10−1 cells of the isolated bacterium for one cells of the pathogen. The effects is ascribed to the inactivation of tolaasin by the live, suppressive bacterial cells, and not to metabolites secreted from the organism into culture media. Examination by conventional bacteriological tests and with testing kits, i.e., MicroStationTMSystem Release 3.5 (Biolog Inc., Hayward, CA), ATB Expression (bioMerieux Inc. Japan) and VITEK (bioMerieux Inc. Japan), failed to assign the organism to any defined bacterial genus. The suppressive bacterium may be useful in future for the development of biocontrol system and/or the construction of genetically modified edible fungi resistant to the disease caused byP. tolaasii.

Key Words

biocontrol agent detoxification Pleurotus ostreatus Pseudomonas tolaasii tolaasins 

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Copyright information

© The Mycological Society of Japan 1998

Authors and Affiliations

  • Takanori Tsukamoto
    • 3
  • Akira Shirata
    • 1
  • Hitoshi Murata
    • 2
  1. 1.Department of SericultureNational Institute of Sericultural and Entomological SciencesTsukubaJapan
  2. 2.Division of Bio-Resource DevelopmentForestry and Forest Products Research InstituteTsukubaJapan
  3. 3.Yokohama Plant Protection ServiceYokohamaJapan

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