Localizing acethyl-serotonin transferase by electron microscopy
N-acethylserotonin, the substrate of hydroxy-indol-o-methyl-transferase (HIOMT) is being synthetized by the serotonin-acethyl-transferase. The localization of the enzyme in the pineal gland has been studied by uranyl and copper ferrocyanide methods. Both methods revealed a mitochondrial localization. The reaction precipitate was situated on the outer and inner membrane as well as in the inner compartment of the mitochondrium. Without acethyl-Co A the reaction failed to take place. Copper ferrocyanide was found more favourable than the uranyl ferrocyanide. No specific reaction, extramitochondrial precipitate occured.
KeywordsMelatonin Pineal Gland Ferrocyanide Potassium Ferricyanide Mitochondrial Localization
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