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Histochemistry

, Volume 46, Issue 2, pp 173–175 | Cite as

Is alcoholic acidic silver nitrate reagent really specific for the histochemical localization of ascorbic acid?

  • Nandita Shah
Short Communication
  • 37 Downloads

Summary

The histochemical localization of ascorbic acid in plant tissues with the alcoholic acidic silver nitrate reagent is shown here to be not specific for ascorbic acid, since some of the polyphenolic substances, including flavonoids, which are known to be widely distributed in plant tissues, are also able to reduce the acidic alcoholic silver nitrate reagent at low temperature (0–4°C) and at pH 2 to 2.5 in dark. This method may perhaps be used for animal tissues where flavonoid pigments do not occur in such large quantities as they do in plants. I therefore, come to the inevitable conclusion that the use of alcoholic acidic silver nitrate reagent in localizing ascorbic acid in plant tissues may be highly misleading.

Keywords

Ascorbic Acid Flavonoid Chalcone Silver Nitrate Fisetin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Chinoy, N. J.: On the specificity of the alcoholic acidic silver nitrate reagent for the histochemical localization of ascorbic acid. Histochemie20, 102–107 (1969)CrossRefGoogle Scholar
  2. Dave, I. C., Saxena, O. P., Abraham, P. G., Pandya, R. B.: Histochemical localization of L-ascorbic acid in plant tissues. Vidya11, 196–206 (1968)Google Scholar
  3. Unni, N. V. M., Shah, C. K.: Ascorbic acid localization during vegetative and reproductive differentiation inCyperus rotundus L. Indian J. Plant Physiol.11, 172–180 (1968)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Nandita Shah
    • 1
    • 2
  1. 1.Botany DepartmentGujarat UniversityAhmedabad-9India
  2. 2.“Upahar” BungalowNear Shreejibag FlatsAhmedabad-9India

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