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Mycoscience

, Volume 38, Issue 4, pp 455–458 | Cite as

A PCR-based method for mating type determination inCochliobolus heterostrophus

  • Abdul Gafur
  • Chihiro Tanaka
  • Seiji Ouchi
  • Mitsuya Tsuda
Short Communication

Abstract

A rapid and accurate method based on multiplex PCR using three different primers was developed for determining mating type inCochliobolus heterostrophus. The primers of MAT113 and MAT123 were uniquely derived fromMAT1-1 andMAT1-2, respectively, and the primer MATcon5 is conserved in the flanking regions of the idiomorphs. The amplification product was estimated to be 702 bp (MAT1-1) or 547 bp (MAT1-2). Crossing experiments confirmed the accuracy of this method, which requires less time than the conventional determination of mating type.

Key Words

Cochliobolus heterostrophus mating type polymerase chain reaction (PCR) 

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Copyright information

© The Mycological Society of Japan 1997

Authors and Affiliations

  • Abdul Gafur
    • 1
  • Chihiro Tanaka
    • 2
  • Seiji Ouchi
    • 1
  • Mitsuya Tsuda
    • 2
  1. 1.Laboratory of Plant Pathology, Faculty of AgricultureKinki UniversityNaraJapan
  2. 2.Laboratory of Environmental Mycoscience, Faculty of AgricultureKyoto UniversityKyotoJapan

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