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Isolation and characterization of two entomopathogenic fungi attackingDiaphorina citri (Homoptera, Psylloidea) in Indonesia

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Mycoscience

Abstract

In an attempt to suppress the propagation of citrus greening disease in Indonesia, we explored pathogens ofDiaphorina citri which vectors the disease. At two orange orchards, manyD. citri adults were found to be dead and covered with fungal mycelia. Two fungi,Paecilomyces fumosoroseus andHirsutella citriformis, were consistently isolated from the infected insects. Molecular phylogeny of their 18S rDNA sequences showed that they belong to the ascomycetous clade of the Clavicipitales/Hypocreales, which embraces many entomopathogenic fungi. When healthy adults ofD. citri were inoculated with conidia of theP. fumosoroseus, the insects died within 6 d.

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Literature cited

  • Aoki, J. 1989. A key for insect pathogenic fungi. Zenkoku Noson Kyoiku Kyokai, Tokyo (In Japanese.)

    Google Scholar 

  • Baker, R. R. and Dunn, P. E. 1990. New directions in biological control. Alan R. Liss, New York.

    Google Scholar 

  • Burges, H. D. 1981. Microbial control of pests and plant diseases 1970–1980. Academic Press, New York.

    Google Scholar 

  • Capoor, S. P., Rao, D. G. and Viswanath, S. M. 1967.Diaphorina citri Kuway, a vector of the greening disease of citrus in India. Indian J. Agric. Sci.37: 572–576.

    Google Scholar 

  • da Graçia, J. V. 1991. Citrus greening disease. Ann. Rev. Phytopathol.29: 109–136.

    Article  Google Scholar 

  • Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution39: 783–791.

    Article  Google Scholar 

  • Jagoueix, S., Bové, J. M. and Garnier, M. 1994. The phloemlimited bacterium of greening disease of citrus is a member of the α subdivision of theProteobacteria. Int. J. Syst. Bacteriol.44: 379–386.

    Article  PubMed  CAS  Google Scholar 

  • Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol.16: 111–120.

    Article  PubMed  CAS  Google Scholar 

  • McClean, A. P. D. and Oberholzer, P. C. J. 1965. Citrus psylla, a vector of the greening disease of sweet orange. S. Afr. J. Agric. Sci.8: 297–298.

    Google Scholar 

  • Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol.4: 406–425.

    PubMed  CAS  Google Scholar 

  • Samson, R. A. 1974.Paecilomyces and some allied Hyphomycates. Stud. Mycol.6: 1–119.

    Google Scholar 

  • Schwartz, R. E., McClean, A. P. D. and Catling, H. D. 1970. The spread of citrus greening disease by the citrus psylla in South Africa: Phytophylactica2: 59–60.

    Google Scholar 

  • Swofford, D. L. 1993. PAUP: phylogenetic analysis using parsimony version 3.1.1.

  • Tanada, Y. and Kaya, H. K. 1993. Insect pathology. Academic Press, San Diego

    Google Scholar 

  • Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res.22: 4673–4680.

    PubMed  CAS  Google Scholar 

  • Tirtawidjaja, S. 1980. Citrus virus research in Indonesia. In “Proceedings of 8th international organization of citrus virologists (IOCV)” (E. C. Calavan, S. M. Garnsey, and L. W. Timmer, Ed.), pp. 129–132. IOCV. Riverside, CA.

    Google Scholar 

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Authors and Affiliations

  1. Department of Entomology and Plant Pathology, Faculty of Agriculture, Gadjah Mada University, 55281, Yogyakarta, Indonesia

    Siti Subandiyah & Franciscus Wagiman

  2. Agency of Industrial Science and Technology, National Institute of Bioscience and Human-Technology, 305-8566, Tsukuba, Ibaraki, Japan

    Naruo Nikoh & Takema Fukatsu

  3. Bio-Oriented Technology Research Advancement Institution, 331-8537, Omiya, Saitama, Japan

    Naruo Nikoh

  4. Insect Pathology Laboratory, Forestry and Forest Products Research Institute, Tsukuba Norin Danchi-nai, P.O. Box 16, 305-0903, Ibaraki, Japan

    Hiroki Sato

  5. Faculty of Agriculture, Shizuoka University, 422-8017, Shizuoka, Japan

    Shinji Tsuyumu

Authors
  1. Siti Subandiyah
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  2. Naruo Nikoh
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  3. Hiroki Sato
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  4. Franciscus Wagiman
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  5. Shinji Tsuyumu
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  6. Takema Fukatsu
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Corresponding author

Correspondence to Takema Fukatsu.

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Subandiyah, S., Nikoh, N., Sato, H. et al. Isolation and characterization of two entomopathogenic fungi attackingDiaphorina citri (Homoptera, Psylloidea) in Indonesia. Mycoscience 41, 509–513 (2000). https://doi.org/10.1007/BF02461672

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  • DOI: https://doi.org/10.1007/BF02461672

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