Theory of RNA-DNA hybridization—I. Stoichiometry at equilibrium

  • John M. Reiner


The equations relating hybridized RNA to free RNA, in the case of simple hybridization, or to ratio of labelled and unlabelled RNA in competitive hybridization, are derived. Analysis of the equations shows how hybridization data may be used to infer properties of the distribution of components in an RNA mixture, or the relation between two distributions in competitive hybridization. A critical examination of the assumptions underlying the equations indicates that some of then may be violated in certain cases, or have no current support, evidential or theoretical. The consequences of such qualifications for the interpretation of hybridization data are indicated.


Initial Slope Hybridization Data Simple Hybridization Competitive Hybridization Cold Mixture 
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Copyright information

© Society for Mathematical Biology 1975

Authors and Affiliations

  • John M. Reiner
    • 1
  1. 1.Department of Biochemistry and PathologyAlbany Medical College of Union UniversityAlbany

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