Mycoscience

, Volume 37, Issue 3, pp 237–241 | Cite as

SSU rDNA sequence support for a close relationship between the elaphomycetales and the Eurotiales and Onygenales

  • Sara Landvik
  • Neil F. J. Shailer
  • Ove E. Eriksson
Original Papers

Abstract

Based on parsimony analyses of eight new SSU rDNA sequences and 24 homologous sequences retrieved from the DNA databases, we suggest a possible phylogenetic relationship of Elaphomycetales with Eurotiales and Onygenales. Our three includedElaphomyces sequences strongly cluster together (bootstrap value 100%) within a monophyletic group (100%) of Elaphomycetales, Eurotiales, and Onygenales. Earlier reports that another cleistothecial lineage (Erysiphe) is related to Leotiales, are supported by our discovery that also another cleistothecial species,Amylocarpus encephaloides, shows affinity to Leotiales. Ascosphaeraceae and Eremascaceae are possibly better accommodated in Onygenales. We describe a new DNA extraction method in which sonication is used to disrupt thick-walled spores. It is useful for both fresh and dried fungal material.

Key Words

cleistothecial ascomycetes DNA extraction Elaphomycetales Plectomycetes SSU rDNA phylogeny 

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Literature cited

  1. Ainsworth, G. C. 1961. Ainsworth and Bisby’s Dictionary of the fungi, 5th ed., CMI, Kew.Google Scholar
  2. Ainsworth, G. C., James, P. W. and Hawksworth, D. L. 1971. Ainsworth and Bisby’s Dictionary of the fungi, 6th ed., CAB, Kew.Google Scholar
  3. Benny, G. L. and Kimbrough, J. W. 1980. A synopsis of the orders and families of Plectomycetes with keys to the genera. Mycotaxon12: 1–91.Google Scholar
  4. Berbee, M. L. and Taylor, J. W. 1992. Two ascomycete classes based on fruiting-body characters and ribosomal DNA sequence. Mol. Biol. Evol.9: 278–284.PubMedGoogle Scholar
  5. Boom, R., Sol, C. J. A., Salimans, M. M. M., Jansen, C. L., Wertheim-van Dillen, P. M. E. and Noordaa van der, J. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol.28: 495–503.PubMedGoogle Scholar
  6. Crumlish, B. and Curran, P. 1994.Amylocarpus encephaloides Currey, a marine fungus new to Ireland. Mycologist8: 83–84.Google Scholar
  7. Currah, R. S. 1985. Taxonomy of the Onygenales: Arthrodermataceae, Gymnoascaceae, Myxotrichaceae and Onygenaceae. Mycotaxon24: 1–216.Google Scholar
  8. Dodge, C. W. 1929. The higher Plectascales. Ann. Mycol.27: 145–184.Google Scholar
  9. Eriksson, O. E. and Hawksworth, D. L. 1993. Outline of the Ascomycetes-1993. Syst. Ascom.,12: 51–257.Google Scholar
  10. Gang, D. R. and Weber, D. J. 1995. Preparation of genomic DNA for RAPD analysis from thick-walled dormant teliospores ofTilletia species. Bio Techniques,19: 92–97.Google Scholar
  11. Grube, M., DePriest, P. T., Gargas, A. and Hafellner, J. 1995. DNA isolation from lichen ascomata. Mycol. Res.99: 1321–1324.Google Scholar
  12. Hawksworth, D. L. 1994. Ascomycete systematics: Problems and perspectives in the Nineties, (ed. by Hawksworth, D. L.), Plenum Press, New York.Google Scholar
  13. Hawksworth, D. L., Sutton, B. C. and Ainsworth, G. C. 1983. Ainsworth & Bisby’s Dictionary of the fungi, 7th ed. CAB, Kew.Google Scholar
  14. Hawksworth, D. L., Kirk, P. M., Sutton, B. C. and Pegler, D. N. 1995. Ainsworth & Bisby’s Dictionary of the fungi, 8th ed., University Press, Cambridge.Google Scholar
  15. Kohlmeyer, J. and Kohlmeyer, E. 1979. Marine mycology: The higher fungi, Academic Press, New York.Google Scholar
  16. Korf, R. P. 1973. Discomycetes and Tuberales. In: The fungi, VI A, (ed. by Ainsworth, G. C., Sparrow, F. K. and Sussman, A. S.), pp. 249–319. Academic Press, New York.Google Scholar
  17. Landvik, S. 1996.Neofecta, a fruitbody-producing genus of the basal ascomycetes, as shown by SSU and LSU rDNA sequences. Mycol. Res.100: 199–202.CrossRefGoogle Scholar
  18. Landvik, S. and Eriksson, O. E. 1994a. Relationships ofTuber, Elaphomyces, andCyttaria (Ascomycotina) inferred from 18S rDNA studies.—In: Ascomycete systematics: Problems and perspectives in the Nineties, (ed. by Hawksworth, D. L.), pp. 225–231. Plenum Press, New York.Google Scholar
  19. Landvik, S. and Eriksson, O. E., 1994b. Relationships of the genusGlaziella (Ascomycota) inferred from 18S rDNA sequences. Syst. Ascom.13: 13–23.Google Scholar
  20. Landvik, S., Eriksson, O. E., Gargas, A. and Gustafsson, P. 1993. Relationships of the genusNeolecta (Neolectales ordor nov. Ascomycotina) inferred from 18S rDNA sequences. Syst. Ascom.11: 107–118.Google Scholar
  21. Luttrell, E. S. 1951. Taxonomy of the pyrenomycetes. Univ. Missouri Studies3: 1–120.Google Scholar
  22. Nannfeldt, J. A. 1932. Studien über die Morphologie und Systematik der nicht-lichenisierten inoperculaten Discomyceten. Nove Acta R. Scient. Upsal., Ser. IV,8: 1–368.Google Scholar
  23. Nishida, H. and Sugiyama, J. 1994. Archiascomycetes: detection of a major new lineage within the Ascomycota. Mycoscience35: 361–366.CrossRefGoogle Scholar
  24. Saccardo, P. A. 1889. Sylloge fungorum omnium hucusque cognitorum, Padova.Google Scholar
  25. Seenz, G. S., Taylor, J. W. and Gargas, A. 1994. 18S rRNA gene sequences and supraordinal classification of the Erysiphales. Mycologia86: 212–216.Google Scholar
  26. Smith, L. S., Lewis, T. L. and Matsui, S. M. 1995. Increased yields of small DNA fragments purified by silica binding. Bio Techniques18: 970–975.Google Scholar
  27. Spatafora, J. W. and Blackwell, M. 1994. The polyphyletic origins of ophiostomatoid fungi. Mycol. Res.98: 1–9.Google Scholar
  28. Swofford D. L. 1991. PAUP: Phylogenetic analysis using parsimony, version 3.0 s.—Computer program distributed by the Illinois Natural History Survey, Champaign, Illinois.Google Scholar
  29. Trappe, J. M. 1979. The orders, families, and genera of hypogeous Ascomycotina (truffels and their relatives) Mycotaxon9: 297–340.Google Scholar
  30. White, T. J., Bruns, T. D., Lee, S. B. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR-Protocols: A guide to methods and applications (ed. by Innis, N., Gelfand, J. and White, T.), pp. 315–322. Academic Press, New York.Google Scholar
  31. Wilmotte, A., Van de Peer, Y., Goris, A., Chapelle, S., De Baere, R., Nelissen, B., Neefs, J. M., Hennebert, S., De Wachter, R. 1993. Evolutionary relationship among higher fungi inferred from small ribosomal subunit RNA sequence analysis. System. Appl. Microbiol.16: 436–444.Google Scholar

Copyright information

© The Mycological Society of Japan 1996

Authors and Affiliations

  • Sara Landvik
    • 1
  • Neil F. J. Shailer
    • 2
  • Ove E. Eriksson
    • 1
  1. 1.Department of Ecological BotanyUmeå UniversityUmeåSweden
  2. 2.Department of Biological AnthropologyUniversity of CambridgeCambridgeUK

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