, Volume 42, Issue 6, pp 627–630 | Cite as

Recovery of degenerate schizostatin production by screening single-basidiospore isolates inSchizophyllum commune

  • Isshin Tanaka
  • Tatsuo Tanimoto
  • Kiyoshi Hamano
  • Tsuyoshi Hosoya
Short Communication


To recover degenerate schizostatin production in aSchizophyllum commune isolate, SANK 17785, we examined schizostatin production of 30 single-basidiospore isolates obtained from a basidiocarp of SANK 17785. One of the isolates showed high productivity (136–154 μg/ml) and maintained its high productivity for 26 mo. To explore other isolates with high schizostatin production, 76 single-basidiospore isolates from 7 wild basidiocarps were obtained and their schizostatin production was quantified. Four of the isolates produced more schizostatin than SANK 17785 did originally.

Key Words

basidiocarp formation genetic recombination Schizophyllum commune schizostatin single-basidiospore isolates 


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Literature cited

  1. Alexopoulos, C. J., Mims, C. W. and Blackwell, M. 1996. Introductory mycology 4th ed., pp. 585–586. John Wiley and Sons, New York.Google Scholar
  2. Butler, G. L. and Pearce, R. B. 1999. Fruiting inducing activity amongst isolates and single spore progeny ofPhellinus contiguus, other species ofPhellinus and certain other wood-rotting fungi. Mycol. Res.103: 482–486.CrossRefGoogle Scholar
  3. Horn, W. S., Simmonds, M. S. J., Schwartz, R. E. and Blaney, W. M. 1996. Variation in production of phomodiol and phomopsolide B byPhomopsis spp. Mycologia88: 588–595.Google Scholar
  4. Moore-Landecker, E. 1996. Fundamentals of the fungi. 4th ed. Prentice-Hall International Limited, London.Google Scholar
  5. Noble, R., Grogan, H. M. and Elliott, T. J. 1995. Variation in morphology, growth and fructification of isolates in theAgaricus subfloccosus complex. Mycol. Res.99: 1453–1461.CrossRefGoogle Scholar
  6. Skerman, V. B. D. 1968. A new type of micromanipulator and microforge. J. Gen. Microbiol.54: 287–297.PubMedGoogle Scholar
  7. Tanimoto, T., Onodera, K., Hosoya, T., Takamatsu, Y., Kinoshita, T., Tago, K., Kogen, H., Fujioka, T., Hamano, K. and Tsujita, Y. 1996. Schizostatin, a novel squalene synthase inhibitor produced by the mushroom,Schizophyllum commune. J. Antibiot.49: 617–623.PubMedGoogle Scholar
  8. Tanimoto, T., Tsujita, Y., Hamano, K. Haruyama, H., Kinoshita, T., Hosoya, T., Kaneko, S., Tago, K. and Kogen, H. 1995. Schizostatin, a potent squalene synthase inhibitor fromSchizophyllum commune: Isolation, structure elucidation, and total synthesis. Tetrahedron Lett.36: 6301–6304.CrossRefGoogle Scholar

Copyright information

© The Mycological Society of Japan 2001

Authors and Affiliations

  • Isshin Tanaka
    • 1
  • Tatsuo Tanimoto
    • 2
  • Kiyoshi Hamano
    • 3
  • Tsuyoshi Hosoya
    • 1
  1. 1.Lead Discovery Research LaboratoriesSankyo Co., Ltd.Tsukuba, Ibaraki-kenJapan
  2. 2.Pharmacology and Molecular Biology Research LaboratoriesSankyo Co., Ltd.Shinagawa, TokyoJapan
  3. 3.Research Information DepartmentSankyo Co., Ltd.Shinagawa, TokyoJapan

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