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Relationship of the genusCordyceps and related genera, based on parsimony and spectral analysis of partial 18S and 28S ribosomal gene sequences

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Mycoscience

Abstract

A molecular phylogenetic study of selected species of three sub-genera of the genusCordyceps was undertaken, along with representatives of the generaAkanthomyces, Aschersonia, Gibellula, Hymenostilbe, Hypocrella, Nomuraea andTorrubiella, to examine their inter-relationship. Phylogenetic analyses of the data indicated that the Clavicipitaceae form a monophyletic group within the Hypocreales, while the monophyly ofCordyceps was not supported. Four clades were identified:Cor. militaris/Cor. pseudomilitaris; Cor. iranginesis/Cor. sphecocephala; Cor. intermedia/Cor. capitata; andCor. cylindrica/Nom. atypicola. The sub-genusNeocordyceps was shown to be monophyletic while the sub-generaEucordyceps andOphiocordyceps do not form monophyletic groups. The genusHypocrella appeared monophyletic, and radiated after the formation of the generaCordyceps, andTorrubiella. Akanthomyces arachnophilus andGi. pulchra, anamorphs ofTorrubiella species, formed a distinct clade that was separate from one formed by the scale insect pathogens,To. luteorostrata andPaecilomyces cinnamomeus, suggesting that this genus may be polyphyletic.

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Literature cited

  • Bremer, K. 1988. The limits of amino acid sequence data in angiosperm phylogenetic reconstruction. Evolution42: 795–803.

    Article  CAS  Google Scholar 

  • Bunyard, B. A., Nicholson, M. S. and Royse, D. J. 1994. A systematic assessment ofMorchella, using RFLP analysis of the 28S ribosomal RNA gene. Mycologia86: 767–772.

    Google Scholar 

  • Cavender, M. A. 1978. Taxonomy with confidence. Mathematical Bioscience40: 271–280.

    Article  Google Scholar 

  • Charleston, M. A. 1998. Spectrum: Spectral analysis of phylogenetic data. Bioinformatics14: 98–99.

    Article  PubMed  CAS  Google Scholar 

  • Charleston, M. A. and Page, D. M. 1999. Spectral analysis—a brief introduction. In: Molecular systematics and plant evolution, (ed. Hollingsworth, P. M., Bateman, R. M. and Gornall, R. J.), pp. 255–267. Taylor and Francis, London.

    Google Scholar 

  • Eriksson, O. E. 1986. Notes on ascomycete systematics. Nos. 1–224. Systema Ascomycetum5: 113–174.

    Google Scholar 

  • Evans, H. C. 1988. Co-evolution of entomogenous fungi and their insect hosts. In: Co-evolution of fungi with plants and animals, (ed. Pirozynski, K. A. and Hawksworth, D. L.), pp. 149. Academic Press, New York.

    Google Scholar 

  • Evans, H. C. and Hywel-Jones, N. L., 1990. Aspects of the generaHypocrella andAschersonia as pathogens, of coccids and whiteflies. In: Proc. 5th Collo. Invertebr. Pathol. Microb. Contl., (ed. Cooper, D. J., Drummond, J. and Pinnock, D. E.), pp. 111–115. Soc. Invertebr. Pathol Adelaide, Australia.

    Google Scholar 

  • Farris, J. S. 1969. A successive approximations approach to character weighting. Syst. Zool.18: 374–385.

    Article  Google Scholar 

  • Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using Bootstrap. Evolution39: 783–791.

    Article  Google Scholar 

  • Gargas, A. and Taylor, J. W. 1992. Polymerase chain reaction (PCR) primers for amplifying and sequencing nuclear 18S rDNA from lichenized fungi. Mycologia84: 589–592.

    CAS  Google Scholar 

  • Glenn, A. E., Bacon, C. W., Price, R. and Hanlin, R. T. 1996. Molecular phylogeny ofAcremonium and its taxonomic implications. Mycologia88: 369–383.

    CAS  Google Scholar 

  • Hawksworth, D. L., Kirk, P. M., Sutton, B. C. and Pegler, D. N. 1995. Ainsworth and Bisby's dictionary of the fungi. Eighth edition. CAB International, the University Press, Cambridge.

    Google Scholar 

  • Hendy, M. D. and Charleston, M. A. 1982. Hadamard conjugation: A versatile tool for modelling sequence evolution. New Zealand. J. Bot.31: 231–237.

    Google Scholar 

  • Hendy M. D. and Penny, D., 1993. Spectral analysis of phylogenetic data. J. Classification10: 5–24.

    Google Scholar 

  • Hultman, T., Stahl, S., Hornes, E. and Uhlén, M. 1989. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids Res.17: 4937–4946.

    PubMed  CAS  Google Scholar 

  • Hywel-Jones, N. L. 1993.Torrubiella luteorostrata a pathogen of scale insects and its association withPaecilomyces cinnamomeus with a note onTorrubiella tenuis. Mycol. Res.97: 1126–1130.

    Google Scholar 

  • Hywel-Jones, N. L. 1994.Cordyceps khaoyaiensis andC. pseudomilitaris, two new pathogens of lepidopteran larvae from Thailand. Mycol. Res.98: 939–942.

    Google Scholar 

  • Hywel-Jones, N. L. 1995a. Notes onCordyceps nutans and its anamorph, a pathogen of hemipteran bugs in Thailand. Mycol. Res.99: 724–726.

    Google Scholar 

  • Hywel-Jones, N. L. 1995b.Cordyceps sphecocephala and aHymenostilbe sp. infecting wasps and bees in Thailand. Mycol. Res.99: 154–158.

    Google Scholar 

  • Hywel-Jones, N. L. 1995c.Hymenostilbe ventricosa sp. nov. a pathogen of cockroaches in Thailand. Mycol. Res.99: 1201–1204.

    Google Scholar 

  • Hywel-Jones, N. L. 1996.Cordyceps myrmecophila-like fungi infection of ants in the leaf litter of tropical forests in Thailand. Mycol. Res.100: 613–619.

    Google Scholar 

  • Hywel-Jones, N. L. and Evans, H. C. 1993. Taxonomy and ecology ofHypocrella discoides and its anamorphAschesonia samoensis. Mycol. Res.97: 871–876.

    Article  Google Scholar 

  • Hywel-Jones, N. L., Goos, R. D. and Jones, E. B. G. (1998).Hirsutella petchabunensis sp. nov. from Thailand, with aHelicoma anamorph. Mycol. Res.102: 577–581.

    Article  Google Scholar 

  • Ibrahim, Y. B. and Low, W. 1993. Potential of mass-production and field efficacy of isolates entomophathogenic fungiBeauveria bassiana andPaecilomyces fumosoroseus againstPlutella xylostella. Int. J. Pest Man.39: 288–292.

    Google Scholar 

  • Kishino, H. and Hasegawa, M. 1989. Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in Hominoidea. J. Mol. Evol.29: 170–179.

    Article  PubMed  CAS  Google Scholar 

  • Kobayasi, Y. 1982. Keys to the taxa of the generaCordyceps andTorrubiella. Trans. Mycol. Soc. Japan23: 329–364.

    Google Scholar 

  • Lee, S. B. and Taylor, J. W. 1990. Phylogeny of five fungus-like protoctistanPhytophthora species, inferred from the internal transcribed spacers of ribosomal DNA. Mol. Biol. Evol.9: 636–653.

    Google Scholar 

  • Link, J. H. F. 1833. Handbuch zur Erkennung der Plize Gewächse.3: 274–286.

    Google Scholar 

  • Miller, J. H. 1949. A revision of the classification of the ascomyces with special emphasis on the Pyrenomycetes. Mycologia41: 99–127.

    Google Scholar 

  • Nannfeldt, J A. 1932. Studien über die Morphologie und Systematik der nichlichenisienten inoperculaten Discomyceten. Novo Acta Regiae Soc. Sci. Upsaliensis8: 1–368.

    Google Scholar 

  • Nikoh, N. and Fukatsu, T. 2000. Interkingdom host jumping underground: Phylogenetic analysis of entomoparastic fungi of the genusCordyceps. Mol. Biol. Evol.17: 629–638.

    PubMed  CAS  Google Scholar 

  • O'Donnell, K. 1993.Fusarium and its near relation. In: The fungal holomorph: mitotic meiotic and pleomorphic speciation in fungal systematics (ed. Reynolds, D. R. and Taylor, J. W.), pp. 223–233. CAB International, Wallingford, U. K.

    Google Scholar 

  • Parkin, J. 1906. Fungi parasitic upon scale insects (Coccidae and Aleyrodidae): a general account with special reference to Ceylon forms. Ann. Royal Bot. Garden, Peradeniya3: 11–82.

    Google Scholar 

  • Patouillard, N. T. 1897. Contributions a la Flore Mycologique du Tonkin. J. Bot.11: 367–370.

    Google Scholar 

  • Petch, T. 1937. Notes on entomogenous fungi. Trans. Brit. Mycol. Soc.21: 34–67.

    Google Scholar 

  • Rambault, A. 1995. Se-Al sequence editor ver 1. d1, Oxford University, UK.

    Google Scholar 

  • Rehner, S. A. and Samuels, G. J. 1995. Molecular systematics of the Hypocreales: a teleomorph gene phylogeny and the status of the anamorphs. Can. J. Bot.73: S816-S823.

    CAS  Google Scholar 

  • Rogerson, C. T. 1970. The Hypocrealean fungi (Ascomycetes Hypocreales). Mycologia62: 865–910.

    PubMed  CAS  Google Scholar 

  • Rossman, A. Y. 1993. Holomorphic Hypocrealean fungi:Nectria sensu stricto and teleomorphs ofFusarium. In: The fungal holomorph: mitotic, meiotic and pleiomorphic speciation in fungal systematics, (ed. Reynolds, D. R. and Taylor J. W.), pp. 149–160. CAB International Wallingford, U.K.

    Google Scholar 

  • Samson, R. A. 1974.Paecilomyces and some allied hypohmycetes. Stud. Mycol. Baarn6: 1–119.

    Google Scholar 

  • Samson, R. A. and Brady, B. L. 1982.Akanthomyces novoguineensis sp. nov. Trans. Brit. Mycol. Soc.79: 571–572.

    Article  Google Scholar 

  • Samson, R. A. and Evans, H. C. 1973. Notes on entomogenous fungi from Ghana I. The generaGibellula andPseudogibellula. Acta Bot. Neerl.22: 522–528.

    Google Scholar 

  • Samson, R. A. and Evans, H. C. 1974. Notes on entomogenous fungi from Ghana. II. The genusAkanthomyces. Acta Bot. Neerl.23: 23–35.

    Google Scholar 

  • Sanger, F., Nicklen, S. and Coulson, A. R. 1977. DNA sequencing with chain terminating inhibitors. Proc. Nat. Acad. Sci. USA74: 5463–5467.

    Article  PubMed  CAS  Google Scholar 

  • Spatafora, J. W. and Blackwell, M. 1993. Molecular systematics of unitunicate perithecial ascomycetes: the Clavicipitales-Hypocreales connection. Mycologia85: 912–922.

    CAS  Google Scholar 

  • Spatafora, J. W., Sung, G.-H., Davies, K. and Hodge, K. T. 2000. Systematics ofCordyceps and the evolution of host shifts. Mycology 2000. British Mycological Society Meeting in Liverpool, April 2000, pp. 20.

  • Swofford, D. L. 1998. PAUP* Phylogenetic analysis using parsimony and other methods. Version 4. b. 2. Sinauer, Sunderland, Mass.

    Google Scholar 

  • Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res.22: 4673–4680.

    PubMed  CAS  Google Scholar 

  • Tzean, S. S., Hsieh, L. S. and Wu, W. J. 1997. Atlas of entomopathogenic fungi from Taiwan. Department of Plant Pathology, National Taiwan University, Council of Agriculture, Taiwan.

    Google Scholar 

  • White, T. J., Bruns, T., Lee, S. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A guide to methods and applications, (ed. Innes, M. A., Gelfand, D. H., Sninsky, J. J. and White, T. J.), pp. 315–322. Academic Press, New York.

    Google Scholar 

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Authors and Affiliations

  1. School of Biological Sciences, University of Portsmouth, King Henry 1st Street, PO 1 2DY, Portsmouth, UK

    Suparp Artjariyasripong & Julian I. Mitchell

  2. BIOTEC-Mycology, Yothi Laboratories, 73/1 Rama 6 Road, 10400, Bangkok, Thailand

    Nigel L. Hywel-Jones & Evan B. Gareth Jones

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  1. Suparp Artjariyasripong
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Artjariyasripong, S., Mitchell, J.I., Hywel-Jones, N.L. et al. Relationship of the genusCordyceps and related genera, based on parsimony and spectral analysis of partial 18S and 28S ribosomal gene sequences. Mycoscience 42, 503–517 (2001). https://doi.org/10.1007/BF02460949

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