Mycoscience

, 39:481 | Cite as

Changes in low molecular weight carbohydrates composition and chitin throughout the cultivation stages ofPleurotus ostreatus

  • Yasushi Obatake
Note

Abstract

Changes in contents of soluble low molecular weight carbohydrates and chitin in a sawdust-rice bran medium during mycelial growth ofPleurotus ostreatus in bottle cultivation were examined in relation to fruit-body yield of nine stocks. Glucose, mannitol, inositol, sucrose, and trehalose were detected in cultures after mycelial spreading. No significant correlation was observed between contents of soluble low molecular weight carbohydrate during mycelial growth and the fruit-body yield. Negative correlation was found between trehalose content in post-harvest cultures and the fruit-body yield. Chitin content in cultures decreased in the fruiting stage. Positive correlation was detected between chitin content of fruit-bodies and the decrement of chitin in post-harvest culture caused by fruit-body growth.

Key Words

bottle cultivation chitin Pleurotus ostreatus sawdust culture soluble low molecular weight carbohydrate 

Literature cited

  1. Braid, G. H. and Line, M. A. 1981. A sensitive chitin assay for the estimation of fungal biomass in hardwood Holzforschung35: 10–15.CrossRefGoogle Scholar
  2. Hashimoto, K. and Takahashi, Z. 1974. Studies on the growth ofPleurotus ostreatus. Mushroom Sci.9: 585–593.Google Scholar
  3. Iwahara, H., Yoshimoto, T. and Fukusumi, T. 1981. Changes in activities of extracellular enzymes during life cycle of the edible mushroom,Pleurotus ostreatus. Mokuzai Gakkaishi27: 331–336. (In Japanese.)Google Scholar
  4. Iwamoto, T., Nakai, M., Sasaki, T. and Naka, H. 1990. β-N-acetylglucosaminidase production by a strain of basidiomycetes and its physiologocal functions. Hakkokogaku68: 107–115. (In Japanese.)Google Scholar
  5. Kazuno, C. and Miura, H. 1985. Chemical constituents ofPleurotus ostreatus. Nippon Shokuhin Kogyo Gakkaishi32: 338–343. (In Japanese.)Google Scholar
  6. Obatake, Y. 1994. Correlation between extracellular enzyme activities and yield of fruit-bodies in several stocks ofPleurotus ostreatus (Jacq.: Fr.) Kummer during growth and fruiting. Bull. Nara Pref. For. Expt. Sta.26: 16–22. (In Japanese.)Google Scholar
  7. Sweely, C. C., Bentley, R., Makita, M. and Wells, W. W. 1963. Gas-liquid chromatography of trimethysilyl derivatives of sugars and related substances. J. Am. Chem. Soc.85: 2497–2507.CrossRefGoogle Scholar
  8. Terashita, T., Ueda, M., Yoshikawa, K., Kono, M. and Shishiyama, J. 1991. Changes in chitin andN-acetylglucosamine contents and chitinolytic enzyme activities during the growth ofFlammulina velutipes fruit-bodies. Nippon Shokuhin Kogyo Gakkaishi39: 827–835.Google Scholar
  9. Tokimoto, K. and Fukuda, M. 1981. Relation between mycelium quantity and fruit-body yield inLentinus edodes bedlogs. Taiwan Mushroom5: 1–5.Google Scholar
  10. Tsuji, A., Kinoshita, T. and Hoshino, M. 1969. Analytical chemical studies on amino sugars. II. Determination of hexosamines using 3-methyl-2-benzothiazolone hydrazone hydrochloride. Chem. Pharm. Bull.17: 1505–1510.PubMedGoogle Scholar
  11. Wessels, J. G. H. 1961. Morphogenesis and biochemical processes inSchizophyllum commune Fr. Wentia23: 1–113.Google Scholar
  12. Yamashita, I., Mori, T., Iino, K., Yanai, S. 1983. Utilization of Job's-tears husk, peanut shell, lawn grass and porous stone for cultivation of oyster mushroom (Pleurotus ostreatus (Jacq. ex Fr) Quel.). Nippon Shokuhin Kogyo Gakkaishi30: 693–697. (In Japanese.)Google Scholar
  13. Yoshida, H., Sugahara, T., Hayashi, J. 1986. Changes in carbohydrates and organic acid during development of mycelium and fruit-bodies of Hiratake mushroom (Pleurotus ostreatus). Nippon Shokuhin Kogyo Gakkaishi33: 519–528. (In Japanese.)Google Scholar
  14. Yoshida, H., Sugahara, T., Hayashi, J. 1987. Changes in the contents of carbohydrates and organic acids in fruit-bodies of Hiratake-mushroom [Pleurotus ostreatus (Fr.) Quel] during development and post-harvest storage. Nippon Shokuhin Kogyo Gakkaishi34: 288–297. (In Japanese.)Google Scholar

Copyright information

© The Mycological Society of Japan 1998

Authors and Affiliations

  • Yasushi Obatake
    • 1
  1. 1.Nara Prefectural Forest Experiment StationTakatori, NaraJapan

Personalised recommendations