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Partial contig map of the smallest chromosome inCoprinus cinereus

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Mycoscience

Abstract

We have constructed a chromosome-specific cosmid library from electrophoretically separated chromosomes of the basidiomyceteCoprinus cinereus and performed contig mapping and analysis of chromosome length polymorphisms (CLPs) for the smallest chromosome of the 5302 strain. A contig map of about 300 kb indicated that the novel size chromosomes in the F1 progeny were apparently recombinants containing physical markers derived from both ends and central regions in this map. This may be the first case in which the formation of CLPs in the F1 generation has been explained using the contig map. The results obtained were consistent with the hypothesis that novel CLPs were produced by meiotic recombination between the parental homologous chromosomes of unequal sizes.

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Literature cited

  • Arima, T. and Morinaga, T. 1995. Inheritance of chromosome length polymorphisms inCoprinus cinereus. Mycoscience36: 9–15.

    Article  Google Scholar 

  • Arima, T., Okida, T. and Morinaga, T. 1996. Behavior of chromosomes after meiosis inCoprinus cinereus. Mycoscience37: 111–115.

    Article  Google Scholar 

  • Bartholdi, M., Meyne, J., Albright, K., Luedemann, M., Cambell, E., Chritton, D., Deaven, L. L. and Cram, L. S. 1987. Chromosome sorting by flow cytometry. In: Methods in enzymology, vol. 151, (ed. by Gottesman, M. M.), pp. 252–267. Academic Press, New York.

    Chapter  Google Scholar 

  • Birnboim, H. C. and Doly, J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res.7: 1513–1523.

    PubMed  CAS  Google Scholar 

  • Boeke, J. D., Eichinger, D. J. and Natsoulis, G. 1991. Doubling Ty1 element copy number inSaccharomyces cerevisiae: host genome stability and phenotypic effects. Genetics129: 1043–1052.

    PubMed  CAS  Google Scholar 

  • Brody, H. and Carbon, J. 1989. Electrophoretic karyotype ofAspergillus nidulans. Proc. Natl. Acad. Sci. USA86: 6260–6263.

    Article  PubMed  CAS  Google Scholar 

  • Brody, H., Griffith, J., Cuticchia, A. J., Arnold, J. and Timberlake, W. E. 1991. Chromosome-specific recombinant DNA libraries from the fungusAspergillus nidulans. Nucleic Acids Res.19: 3105–3109.

    PubMed  CAS  Google Scholar 

  • Casselton, L. A. 1995. Genetics ofCoprinus. In: The mycota II: genetics and biotechnology, (ed. by Kuck, U.), pp. 35–48. Springer-Verlag, Berlin.

    Google Scholar 

  • Chu, G., Vollrath, D. and Davis, R. W. 1986. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science234: 1582–1585.

    PubMed  CAS  Google Scholar 

  • Coleman, M. J., McHale, M. T., Arnau, J., Watson, A. and Oliver, R. P. 1993. Cloning and characterization of telomeric DNA fromCladosporium fulvum. Gene132: 67–73.

    Article  PubMed  CAS  Google Scholar 

  • Coulson, A., Sulston, J., Brenner, S. and Karn, J. 1986. Toward a physical map of the genome of the nematodeCaenorhabditis elegans. Proc. Natl. Acad. Sci. USA83: 7821–7825.

    Article  PubMed  CAS  Google Scholar 

  • Davis, C. R., Kempainen, R. R., Srodes, M. S. and McClung, C. R. 1994. Correlation of the physical and genetic maps of the centromeric region of the right arm of linkage group III ofNeurospora crassa. Genetics136: 1297–1306.

    PubMed  CAS  Google Scholar 

  • Dotzlaf, J. E. and Yeh, W. K. 1987. Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase fromCephalosporium acremonium. J. Bacteriol.169: 1611–1618.

    PubMed  CAS  Google Scholar 

  • Dutta, S. K. 1974. Repeated DNA sequences in fungi. Nucleic Acids Res.11: 1411–1419.

    Google Scholar 

  • Farman, M. L. and Leong, S. A. 1995. Genetic and physical mapping of telomeres in the rice blast fungus,Magnaporthe grisea. Genetics140: 479–492.

    PubMed  CAS  Google Scholar 

  • Francis, D. M. and Michelmore, R. W. 1993. Two classes of chromosome-sized molecules are present inBremia lactucae. Exp. Mycol.17: 284–300.

    Article  CAS  Google Scholar 

  • Holm, P. B., Rasmussen, S. W., Zickler, D., Lu, B. C. and Sage, J. 1981. Chromosome pairing, recombination nodules and chiasma formation in the basidiomyceteCoprinus cinereus. Carlsberg Res. Commun.46: 305–346.

    Google Scholar 

  • Hulbert, S. H., Ilott, T. W., Legg, E. J., Lincoin, S. E., Lander, E. S. and Michelmore, R. W. 1988. Genetic analysis of the fungus,Bremia lactucae, using restriction fragment length polymorphisms. Genetics120: 947–958.

    PubMed  CAS  Google Scholar 

  • Kohara, Y., Akiyama, K. and Isono, M. 1987. The physical map of the wholeE. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell50: 495–508.

    Article  PubMed  CAS  Google Scholar 

  • Krumlauf, R. and Marzluf, G. A. 1979. Characterization of the sequence complexity and organization of theNeurospora crassa genome. Bichemistry18: 3705–3713.

    Article  CAS  Google Scholar 

  • Leskiw, B. K., Aharomowitz, Y., Mevarech, M., Wolfe, S., Vining, L. C., Westlake, D. W. and Jensen, S. E., 1988. Cloning and nucleotide sequence determination of the isopenicillin N synthetase gene fromStreptomyces clavuligerus. Gene62: 187–196.

    Article  PubMed  CAS  Google Scholar 

  • Louis, E. J., Naumova, E. S., Lee, A., Naumov, G. and Haber, J. E. 1994. The chromosome end in yeast: its mosaic nature and influence on recombinational dynamics. Genetics136: 789–802.

    PubMed  CAS  Google Scholar 

  • Maniatis, T., Fritsch, E. F. and Sambrook, J. 1982. Molecular cloning, a laboratory manual. Cold Spring Harbor, New York.

  • Mautino, M. R., Haedo, S. D. and Rosa, A. L. 1993. Physical mapping of meiotic crossover events in a 200-kb region ofNeurospora crassa linkage group I. Genetics134: 1077–1083.

    PubMed  CAS  Google Scholar 

  • McCormick, M. K., Shero, J. H., Cheung, M. C., Kan, Y. W., Hieter, P. A. and Antonarakis, S. E. 1989. Construction of human chromosome 21-specific yeast artificial chromosomes. Proc. Natl. Acad. Sci. USA86: 9991–9995.

    Article  PubMed  CAS  Google Scholar 

  • NIH/CEPH Collaborative Mapping Group. 1992. A comprehensive genetic linkage map of the human genome. Science258: 67–86.

    Google Scholar 

  • North, J. 1987. In: Genetic maps 1987, vol. 4, (ed. by O'Brian, S. J.), pp. 340–345. Cold Spring Harbor Laboratory Press, New York.

    Google Scholar 

  • O'Connell, P., Leach, R. J., Ledbetter, D. H., Cawthon, R. M., Culver, M., Eldridge, J. R., Frej, A. K., Holm, T. R., Wolff, E., Thayer, M. J., Schafer, A. J., Fountain, J. W., Wallace, M. R., Collins, F. S., Skolnick, M. H., Rich, D. C., Journier, R. E. K., Baty, B. J., Carey, J. C., Leppert, M. F., Lathrop, G. M., Lalouel, J. M. and White, R. 1989. Fine structure DNA mapping studies of the chromosomal region harboring the genetic defect in neurofibromatosis type I. Am. J. Hum. Genet.44: 51–57.

    PubMed  Google Scholar 

  • Oliver, S. G. et al. 1992. The complete DNA sequence of yeast chromosome III. Nature357: 38–46.

    Article  PubMed  CAS  Google Scholar 

  • Orbach, M. J., Vollrath, D., Davis, R. W. and Yanofsky, C. 1988. An electrophoretic karyotype ofNeurospora crassa. Mol. Cell Biol.8: 1469–1473.

    PubMed  CAS  Google Scholar 

  • Parsons, K. A., Chumley, F. G. and Valent, B. 1987. Genetic transformation of the fungal pathogen responsible for rice blast disease. Proc. Natl. Acad. Sci. USA84: 4161–4165.

    Article  PubMed  CAS  Google Scholar 

  • Rommens, J. M., Iannuzzi, M. C., Kerem, B., Drumm, M. L., Melmer, G., Kean, M., Rozmahel, R., Cole, J. L., Kennedy, D., Hidaka, N., Zsiga, M., Buchwald, M., Riordan, J. R., Tsui, L. and Collins, F. S. 1989. Identification of the cystic fibrosis gene: Chromosome walking and jumping. Science245: 1059–1065.

    PubMed  CAS  Google Scholar 

  • Samson, S. M., Belagaje, R., Blankenship, D. T., Chapman, J. L., Perry, D., Skatrud, P. L., VanFrank, R. M., Abraham, E. P., Baldwin, J. E., Queener, S. W. and Ingolia, T. D. 1985. Isolation, sequence determination and expression inEscherichia coli of the isopenicillin N synthetase gene fromCephalosporium acremonium. Nature318: 191–194.

    Article  PubMed  CAS  Google Scholar 

  • Schechtman, M. G. 1990. Characterization of telomere DNA fromNeurospora crassa. Gene88: 159–165.

    Article  PubMed  CAS  Google Scholar 

  • Smith, C. L., Econome, J. G., Schutt, A., Klco, S. and Cantor, C. R. 1987. A physical map of theEscherichia coli K12 genome. Science236: 1448–1453.

    PubMed  CAS  Google Scholar 

  • Timberlake, W. E. 1978. Low repetitive DNA content inAspergillus nidulans. Science202: 973–975.

    PubMed  CAS  Google Scholar 

  • Viskochil, D., Buchberg, A. M., Xu, G., Cawthon, R. M., Stevens, J., Wolff, R. K., Culver, M., Carey, J. C., Copeland, N. G., Jenkins, N. A., White, R. and O'Connell, P. 1990. Deletions and a transiocation interrupt a cloned gene at the neurofibromatosis type 1 locus. Cell62: 187–192.

    Article  PubMed  CAS  Google Scholar 

  • Wallace, R. W., Marchuk, D. A., Andersen, L. B., Letcher, R., Odeh, H. M., Saulino, A. M., Fountain, J. W., Brereton, A., Nicholson, J., Mitchell, A. L., Brownstein, B. H. and Collins, F. S. 1990. Type 1 neurofibromatosis gene: identification of a large transcript disrupted in three NF 1 patients. Science249: 181–186.

    PubMed  CAS  Google Scholar 

  • Wilke, C. M., Maimer, E. and Adams, J. 1992. The population biology and evolutionary significance of Ty elements inSaccharomyces cerevisiae. Genetica86: 155–173.

    Article  PubMed  CAS  Google Scholar 

  • Zolan, M. E. 1995. Chromosome-length polymorphism in fungi. Microbiol. Rev.59: 686–698.

    PubMed  CAS  Google Scholar 

  • Zolan, M. E., Crittenden, J. R., Heyler, N. K. and Seitz, L. C. 1992. Efficient isolation and mapping ofrad genes of the fungusCoprinus cinereus using chromosome-specific libraries. Nucleic Acids Res.20: 3993–3999.

    PubMed  CAS  Google Scholar 

  • Zolan, M. E., Heyler, N. K. and Stassen, N. Y. 1994. Inheritance of chromosome-length polymorphisms inCoprinus cinereus. Genetics137: 87–94.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Kagamiyama 1-4-1, 739, Higashi-Hiroshima, Japan

    Toshihide Arima, Nobuhiro Tagashira & Tsutomu Morinaga

  2. Department of Biology, Faculty of Science, Okayama University, 700, Okayama, Japan

    Takashi Kamada

  3. Center for Gene Science, Hiroshima University, Kagamiyama 1-4-2, 739, Higashi-Hiroshima, Japan

    Nobukazu Tanaka

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  1. Toshihide Arima
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  2. Nobuhiro Tagashira
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  3. Takashi Kamada
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  4. Nobukazu Tanaka
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  5. Tsutomu Morinaga
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Arima, T., Tagashira, N., Kamada, T. et al. Partial contig map of the smallest chromosome inCoprinus cinereus . Mycoscience 38, 127–133 (1997). https://doi.org/10.1007/BF02460847

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