Early stages in the mechanism of action of glucocorticoids on human platelets. Effect of hydrocortisone on intracellular cAMP and Ca2+ content
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Changes in basal and stimulated levels of cAMP and calcium induced by hydrocortisone in a wide range of concentration (0.1–25 μM) are studies in a suspension of washed human platelets. The effects of hydrocortisone on the activity of preparations modulating various stages of the adenylate cyclase system (forskolin, adenosine, adrenaline, and 3-isobutyl-1-methylxanthine) are compared. Platelets are stimulated with collagen, platelet activating factor, and thapsigargin. Hydrocortisone in different concentrations acts as both activator and inhibitor of calcium metabolism in platelets.
Key Wordsplatelets calcium cAMP hydrocortisone thapsigargin inhibitors of platelet activation
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