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Early stages in the mechanism of action of glucocorticoids on human platelets. Effect of hydrocortisone on intracellular cAMP and Ca2+ content

  • Pharmacology and Toxicology
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

Changes in basal and stimulated levels of cAMP and calcium induced by hydrocortisone in a wide range of concentration (0.1–25 μM) are studies in a suspension of washed human platelets. The effects of hydrocortisone on the activity of preparations modulating various stages of the adenylate cyclase system (forskolin, adenosine, adrenaline, and 3-isobutyl-1-methylxanthine) are compared. Platelets are stimulated with collagen, platelet activating factor, and thapsigargin. Hydrocortisone in different concentrations acts as both activator and inhibitor of calcium metabolism in platelets.

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Authors and Affiliations

  1. Department of Pharmacology and Radiobiology, Russian State Medical University, Moscow

    P. V. Sergeev, A. S. Dukhanin & F. R. Gubaeva

Authors
  1. P. V. Sergeev
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  2. A. S. Dukhanin
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  3. F. R. Gubaeva
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 123, No. 6, pp. 663–665, June, 1997

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Sergeev, P.V., Dukhanin, A.S. & Gubaeva, F.R. Early stages in the mechanism of action of glucocorticoids on human platelets. Effect of hydrocortisone on intracellular cAMP and Ca2+ content. Bull Exp Biol Med 123, 577–579 (1997). https://doi.org/10.1007/BF02458082

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  • DOI: https://doi.org/10.1007/BF02458082

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