Summary
Water proton spin-lattice relaxation and self-diffusion in aqueous solutions of human serum albumin have been studied by1H NMR as a function of the protein concentration. Spin-lattice relaxation data, which display a nonlinear behaviour with the protein concentration, could be fitted with a two-phase model taking into account the experimentally determined hydration («bound») water values. Despite a similar trend is registered for the water self-diffusion coefficient, such a model has been found unable to explain the related experimental data taken as a function of the biomolecule concentration. On the other hand, the solute-induced proton self-diffusion decrease could be satisfactorily interpreted by postulating an enhanced probability of hydrogen-bond formation occurring within the «vicinal» water surrounding the biomolecules for several hydration shells. The consistency within the two models is discussed in connection with the magnetic interactions occurring within the solute-solvent systems.
Similar content being viewed by others
References
F. Franks andS. Mathias (Editors):Biophysics of Water (J. Wiley & Sons, Chichester, 1982).
International Conference on the Microphysics of Enzime Catalysis, Il Ciocco 5–7 October, 1987, Book of Abstracts.
I. D. Kunts andW. Kauzmann:Adv. Protein Chem.,28, 239 (1974).
G. Schauer, R. Kimmich andW. Nusser:Biophys. J.,53, 397 (1988).
C. F. Polnaszek andR. G. Bryant:J. Am. Chem. Soc.,106, 429 (1984).
B. M. Fung:Biophys. J.,18, 23 (1977).
W. M. Shirley andR. G. Bryant:J. Am. Chem. Soc.,104, 2910 (1982).
E. R. Andrew, D. J. Bryant andE. M. Cashell Chem. Phys. Lett.,69, 55 (1980).
E. R. Andrew, D. J. Bryant andT. Z. Ritzvi:Chem. Phys. Lett.,95, 463 (1983).
P. Noto, M. Migliore, F. Sciortino andS. L. Fornili:Mol. Simulation,1, 225 (1988).
C. R. Cantor andP. R. Schimmel:Biophysical Chemistry, Part II (Freeman and Comp., San Francisco, Cal., 1980).
W. Doster, A. Bachleitner, R. Dunau, M. Hiebl andE. Luscher:Biophys. J.,50, 213 (1986).
F. M. Etzler andW. Drost-Hansen:Croatica Chem. Acta,56, 563 (1983) and references therein.
H. E. Stanley andJ. Teixeira:J. Chem. Phys.,73, 3404 (1980).
P. B. Macedo andT. A. Litovitz:J. Chem. Phys.,42, 245 (1965).
T. Gillen, D. C. Douglass andJ. R. Hoch:J. Chem. Phys.,57, 5117 (1972).
F. P. Ricci, M. A. Ricci andD. Rocca:J. Chem. Phys.,66, 5509 (1977).
A. Angell:J. Phys. Chem.,75, 3698 (1971).
M. H. Cohen andD. Turnbull:J. Chem. Phys.,51, 1164 (1954).
P. G. Morris:Nuclear Magnetic Resonance Imaging in Medicine and Biology (Claredon Press, Oxford, 1986).
S. Conti:Mol. Phys.,59, 449 (1986).
S. H. Koenig andW. E. Schillinger:J. Biol. Chem.,244, 3283 (1969).
K. Hallenga andS. H. Koenig:Biochemistry,15, 4255 (1976).
J. Oakes:J. Chem. Soc. Far. I,72, 216 (1976).
S. H. Koenig, R. G. Bryant, K. Hallenga andG. S. Jacob:Biochemisty,17, 4348 (1978).
M. Eisenstadt:Biochemistry,24, 3407 (1985).
H. E. Rorshach andC. F. Hazlewood:J. Magn. Reson.,70, 79 (1986).
J. H. Wang, C. B. Anfinsen andF. M. Polestra:J. Am. Chem. Soc.,76, 4763 (1954).
J. H. Wang:J. Am. Chem. Soc.,76, 4755 (1954).
D. Bourret andJ. Parello:J. Phys.,45, 255 (1984).
O. F. Bezrukov andB. P. Nikolaev Strukt. Rol Vody Zhivom Org.,3, 58 (1970).
W. Brown andP. Stilbs:Chem. Scripta,19, 161 (1982).
J. R. Zimmerman andP. Stilbs:J. Phys. Chem.,61, 1328 (1957).
F. M. Etzler:J. Coll. Interf. Sci.,92, 43 (1983).
R. Lamanna andS. Cannistraro:Chem. Phys. Lett.,164, 563 (1989).
P. Marzola andS. Cannistraro:Physiol. Chem. Phys. Med. NMR,18, 263 (1986).
M. Sass andD. Zeissow:J. Magn. Reson.,20, 263 (1977).
E. L. Hahn:Phys. Rev.,80, 580 (1950).
H. Y. Carr andE. M. Purcell:Phys. Rev.,94, 630 (1954).
R. Mills:J. Phys. Chem.,77, 5 (1973).
H. C. Torrey:Phys. Rev.,104, 563 (1956).
J. R. C. Van der Marel andJ. De Bleijser:Chem. Phys. Lett.,141, 251 (1987).
F. H. Stillinger:Science,209, 451 (1980).
W. Drost-Hansen: in ref. [1],. p. 163.
S. Cannistraro andF. Sacchetti:Phys. Rev. A,33, 745 (1986).
G. Giugliarelli andS. Cannistraro:Appl. Magn. Res.,1, 85 (1990).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lamanna, R., Cannistraro, S. 1H NMR study of water relaxation and self-diffusion in human serum albumin aqueous solutions. Il Nuovo Cimento D 13, 261–271 (1991). https://doi.org/10.1007/BF02456954
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02456954